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Adenylate

Adenylate

salt or ester of adenylic acid.


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cAMP 2nd Messenger Pathway and Experiments

... are only believed to have no adenylyl cyclase present) may actually have adenylyl cyclase present, or that there is a different class of the adenylate cyclase that can act in the place of the adenylyl cyclase if it is indeed absent from the cells. I'd take a good guess and assume that the ...

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by mkay28
Wed Sep 25, 2013 3:14 pm
 
Forum: Cell Biology
Topic: cAMP 2nd Messenger Pathway and Experiments
Replies: 2
Views: 1225

cAMP 2nd Messenger Pathway and Experiments

... it mutated in the cyc- cells, but I guess they do not know that), so it's little hard to propose something. Anyway, there are several classes of adenylate cyclases, so they probably eliminated only one gene while others were unaffected. Moreover, they do not know for sure whether cyc- cells were ...

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by JackBean
Wed Sep 25, 2013 11:21 am
 
Forum: Cell Biology
Topic: cAMP 2nd Messenger Pathway and Experiments
Replies: 2
Views: 1225

cAMP 2nd Messenger Pathway and Experiments

... ATP, GTP, and Mg2+ were included in mixture. Mixture was: cell membranes from the cyc- cells, protein extracts from cyc+ cells with inactivated adenylate cyclase, and hormone, GTP, ATP, and Mg2+. Results: upon hormonal stimulation, cAMP was still produced. A. If experiment 1 was the only experiment ...

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by mkay28
Tue Sep 24, 2013 10:43 pm
 
Forum: Cell Biology
Topic: cAMP 2nd Messenger Pathway and Experiments
Replies: 2
Views: 1225

Re: T7 Expression System

... The use of blue-sephadex, Ap5A elution and a second G-100 sephadex separation yield homogenous adenylate kinase in E.Coli anyway (the putative gene i'm dealing with show's a high degree of similarity in sequence to E.Coli AK). However this would ...

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by TjCooper
Tue Jan 03, 2012 1:22 pm
 
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 2979

Re: T7 Expression System

... the system at hand which includes no usable purification tag. I guess I will run it through a blue-sephadex affinity column (as it's a putative adenylate kinase) and see where it goes from there!

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by TjCooper
Tue Jan 03, 2012 11:55 am
 
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 2979
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