Addition reaction

1. A reaction has been design to be carried out in 4ml total volume. It requires a final phenylalanine concentration of 5mM. If the protocol allows 50 ul for the phenylalanine addition, what concentration would you make up the stock solution? 2.A particular cell line will ...

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Yes, I have tried both the same temperature in addition to trying slightly lower and slightly higher. No difference. But as my standard reaction goes I use the same as the temperature published.

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... of the stabilised diluted plasma, 0.15mM NADH and 1mM pyruvate. The reaction was carried out at 25oC and started by adding the pyruvate last. The following readings were obtained: Time after pyruvate addition/min Absorbance at 340nm 0 0.98 1 0.82 2 0.66 3 0.50 4 0.40 5 0.35 6 ...

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... Q: The enzyme deoxynucleotidyl terminal transferase (TdT) catalyses the addition of dNTPs onto the 3’ ends of double stranded DNA molecules. In an ... enzyme Alu I. (Lambda DNA is cut 143 times with Alu I). The following reaction was set up: 17ul H2O (ul = microlitres) 6ul 5x tailing buffer 2ul ...

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... because all the enzymes are saturated and are already catalyzing reactions as fast as they can. 8. Assuming there is excess substrate that enzyme is not colliding with, an addition of enzyme after 100 seconds would increase the rate of reaction until the ...

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