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Active

Active

Characterised by action, not passive, not expectant.


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principle of error-prone PCR???

... it is obviously not the charges, Mn2+ ions carry two positive as well as magesium; plus, Mn2+ ions have exactly the same function with Mg2+, to active enzymes. So I am thinking that Mg2+ & Mn2+ combined to maximum the Taq enzyme activities, leave it no time to proofread.

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by feixiaorou
Wed Oct 01, 2014 3:51 am
 
Forum: Molecular Biology
Topic: principle of error-prone PCR???
Replies: 2
Views: 276

Re: Any ideas for vaccine development or anti-viral drugs???

... female subjects between 18 and 50 years of age were enrolled in 6 dose escalation cohorts of 5 subjects each and received a single IV infusion of active study drug (0.005, 0.05, 0.5, 1.5, 3, and 4.5 mg/kg per component) or placebo in a 4:1 ratio. Both AVI-6002 and AVI-6003 were safe and well tolerated ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 479

mRNA vs. cell reporter assay

... Maybe the reporter system provides us with more information than RT-PCR/Western blot, because it proves that the transcription factor is actually active too, not just present? I thought it is enough to detect a significant increase in the expression by Western blot and we could conclude that the ...

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 694

Recessive and dominant genetics - Pitx1 case study

... because the trait is recessive, however i am not sure? Is there anyone to shed light on this for me? The 2nd question in this reading is involving active and in active genes Which regions in the Pitx1 gene complex (below) do you expect to be functional and which regions are non-functional in manatees ...

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by scienceguy914
Mon Sep 08, 2014 1:44 am
 
Forum: Genetics
Topic: Recessive and dominant genetics - Pitx1 case study
Replies: 0
Views: 426

BRC-ALT Tyrosine Kinase

... BRC-ALT tyrosine Kinase is a problem in leukemia because it is transcribed more often when on Chromosome 22 or if it is simply just always in the active configuration due the mutation, or both? I have looked on access medicine and watched Kevin Ahern's lectures on youtube and both causes are mentioned, ...

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by tmbirkhead
Thu Aug 14, 2014 1:22 am
 
Forum: Molecular Biology
Topic: BRC-ALT Tyrosine Kinase
Replies: 0
Views: 324
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