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Actinomycin D

This substance is sometimes used as an antibiotic, as it has the capability of inhibiting the transcription of mrna from dna. This means that in the case of viral attack, actinomycin D could inhibit the replication of a virus by stopping protein synthesis (the food supply it needs) in the very cells the virus is trying to affect.


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Actinomycin D treatment of cells

Hi, I am trying to use actinomycin D and/or alpha-amanitin to inhibit transcription in mouse embryonic stem cells. I would appreciate if someone could share his/her experience on the concentrations of drugs and cells to use for treatment, ...

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by minerva
Sun Jun 13, 2010 1:34 pm
 
Forum: Molecular Biology
Topic: Actinomycin D treatment of cells
Replies: 1
Views: 2090

mRNA stability assay by actinomycin

Hi I want to study the stability of mRNA by adding Actinomycin D on transfected cell line. What is the suitable time to treat the transfected cells with Actinomycin to inhibit RNA synthesis? Is it after 24 hrs posttransfection or more? I did a first ...

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by roniadam
Fri Nov 27, 2009 11:12 pm
 
Forum: Molecular Biology
Topic: mRNA stability assay by actinomycin
Replies: 0
Views: 3293

Enquiry about interpreting the FACS result

... such as cytokines, interleukines etc.) and let the cells to "calm down". If you are unsure about dead cells, you may try using 7-Amino-actinomycin D (7-AAD) [trade name BD Via-Probe] to exclude dead cells on FACSCanto. Of course, simply using some other cells can do the tirck as well.

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by biohazard
Wed Feb 04, 2009 1:00 pm
 
Forum: Cell Biology
Topic: Enquiry about interpreting the FACS result
Replies: 4
Views: 4944


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