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Actin n-acetylaminopeptidase

Actin n-acetylaminopeptidase

(Science: enzyme) catalyses the amino-terminal processing of actin by removal of acetylmethionine from the amino terminus for class i actins or the removal of the second residue as an n-acetylamino acid for class ii actins

registry number: EC 3.4.11.-


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myosin and actin constituent amino acids?

Do we know what the constituent amino acids of myosin and actin are, and the ratios of these amino acids in muscle tissue? Thank you.

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by mattw
Sat Aug 24, 2013 7:58 pm
 
Forum: Human Biology
Topic: myosin and actin constituent amino acids?
Replies: 1
Views: 680

Problem with the normalization of PCR Ct values

in my case when I have a gene of interest and the standard gene (usually actin) I divide the calculated conc. in fmol of the gene of interest by the calculated conc. of actin. about the ct values, the higher the value, less copies are there, if you make a standard ...

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by bravebeaker
Wed May 01, 2013 1:12 am
 
Forum: Molecular Biology
Topic: Problem with the normalization of PCR Ct values
Replies: 5
Views: 3679

RT PCR contamination.

... that I have tried that have improved this situation. My supervisor suggest maybe there is contamination of the amplicon I am testing. We use b-actin as a control for a lot of experiments so it is plausible but I find it difficult to believe when in my endpoint PCR I got the negative for that ...

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by georgewiggins
Thu Sep 06, 2012 9:49 pm
 
Forum: Molecular Biology
Topic: RT PCR contamination.
Replies: 2
Views: 4053

Idea to determine order of assembly of spliceosome

... that you could have false results because of one-case-only binding/non-binding. To explain - imagine you will design probes for intron in gene for actin. Generaly there are five proteins in spliceosome, let's call them S pliceosome P rotein1 - 5, but in actin is not present SP3, but are present ...

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by JackBean
Wed May 30, 2012 8:06 am
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7565

Re: Please identify this structure. THX.

BTW, It doesn't co-localize with F-actin and microtubes. It seems look like some kind of vesicles. But these vesicles don't like endosomes, golgi or ER, too.

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by fangpaul74
Fri Feb 24, 2012 3:50 pm
 
Forum: Cell Biology
Topic: Please identify this structure. THX.
Replies: 3
Views: 1850
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