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Acrosome

Acrosome

(Science: cell biology) a membrane-bound compatment at the tip of the head of a sperm which contains lytic enzymes (hyaluronidase in mammalian sperm) which digest the outer surface of the egg and allow the sperm to inject its haploid dna.

The compartment is secreted by the golgi apparatus of the spermatid.

Origin: gr. Soma = body a process at the anterior end of a sperm Cell that produces enzymes to facilitate penetration of the egg.Found at the head of a sperm gamete, the acrosome is an enzyme filled sac that assists in breaking down the wall of the egg mother cell so that the sperm can fertilise the egg.


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Mammalian Gametes

Basically then, the male gamete has alot of its cytoplasm reduced with a big huge vesicle called the acrosome that contains the enzymes to eat thru the outer slimy layer that surrounds the egg cell. It has concentrated all of its mitocondria to the end where there is the ...

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by kolean
Wed Sep 02, 2009 10:41 pm
 
Forum: Cell Biology
Topic: Mammalian Gametes
Replies: 3
Views: 51

Spermatozoa staining

... their viability/vitality. I read that double-staining (trypan blue/congo red + Giemsa) is good to differ dead/alive and also the presence of acrosome. Triple-staining (eosin/nigrosin+Bismarck brown+...(I forgot the third dye)) is said good too, but I don't have all the component. Well, the ...

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by DoctorStein
Sun Jun 29, 2008 11:14 am
 
Forum: Cell Biology
Topic: Spermatozoa staining
Replies: 0
Views: 532

Evolution of reproduction: Mobility of cells

... i have no time to explain that now. maybe later or tomorrow. Strange old world isn't it? How and why is the sperm adapted with enzymes in the acrosome and with various glandular fluids for a location which it has never visited befoe? I'd love to find out the answer.

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by Heronumber0
Sat Jul 07, 2007 6:46 pm
 
Forum: Evolution
Topic: Evolution of reproduction: Mobility of cells
Replies: 6
Views: 1604

has anyone tried the acrosome reaction in vitro?

... to induce the axrosome reaction in vitro, and to check whether they are reacted or not, I stain with FITC-PNA, which should only stain intact acrosomes. I have looked through all the papers, the entire internet, and asked a couple of dusty old professors, but noone has given me a decent protocol, ...

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by deedeesofia
Wed Aug 31, 2005 8:02 pm
 
Forum: General Discussion
Topic: has anyone tried the acrosome reaction in vitro?
Replies: 0
Views: 545


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