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Acridine

Acridine

10-azaanthracene;a dye, dye intermediate, and antiseptic precursor (9-aminoacridine, acriflavine, proflavine hemisulfate derived from coal tar and irritating to skin and mucous membranes.

Synonym: dibenzopyridine.


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Determination of Mitochondrial Mass

Can somebody help me with the best way to determine mitochondrial mass? I am thinking to use NAO (Acridine Orange 10-nonyl bromide) but I can´t find the protocol, eg. number of cells needed, concentrations, etc. Does someone have the protocol to do this? Thanks a lot! Lidia

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by Lidia
Wed Sep 30, 2009 5:51 pm
 
Forum: Cell Biology
Topic: Determination of Mitochondrial Mass
Replies: 0
Views: 695

How to differentiate between RNA and DNA

... intense and raise the background staining in the entire well. I think there are some RNA-specific dyes around—Northerns were once stained with acridine orange(?) or something like that if I remember correctly. You can do the 260/280 ratio thing, but it is often hard to interpret. The rule of ...

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by blcr11
Thu Sep 13, 2007 5:13 pm
 
Forum: Molecular Biology
Topic: How to differentiate between RNA and DNA
Replies: 12
Views: 9246

The Fiber Disease

... EDB and visualized in the transmission electron microphotograph illustrated in this paper. The spherical bodies showed fluorescent labeling with acridine orange and with ethidium bromide supporting the idea that they contained nucleic acids. TMRE (tetramethyl rhodamine ethyl ester), a mitochondrion-specific ...

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by RANDY
Fri Dec 22, 2006 4:55 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5481678


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