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Acetylation

Acetylation

formation of an acetyl derivative.


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Theories - Origin of Life

... far stronger than “appears to be” – I would just use “is”). There are lots of epigenetic mechanisms, not just “an” epigenetic mechanism ‒ histone acetylation, histone methylation, cytosine methylation, etc. Undifferentiated cells also have epigenetic mechanisms. This mechanism regulates the position ...

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by AstraSequi
Tue Mar 27, 2012 2:41 am
 
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 560
Views: 890024

Re: Sense effect of RNAi

... to Argonaute can affect transcription. I recall reading that Argonaute-RNA complexes can direct changes in methylation of RNA (or was it histone acetylation? It's been a few years since I read that). Either way, by changing the epigenetic state of a gene the Argonaute-RNA complex can change the ...

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by jonmoulton
Tue Feb 08, 2011 4:01 pm
 
Forum: Molecular Biology
Topic: Sense effect of RNAi
Replies: 9
Views: 4307

Re: Primer design in the promoter region

... of my genes is changed because of the cDNA microarray and subsequent QRT-PCRs and Western blots. Now I want to know if it is because of altered acetylation on the promoter region itself or other mechanisms. Thats why I am using ChIP to extract the regions that have an altered acetylation pattern ...

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by ChIP
Sun Aug 15, 2010 7:25 pm
 
Forum: Molecular Biology
Topic: Primer design in the promoter region
Replies: 10
Views: 11118

Primer design in the promoter region

... against acetylated histone h3. I did a cDNA microarray before and now I want to check if the genes I found there are really regulated by histone acetylation. Thats why I want to do quantitative realtime PCR after the ChIP. The problem is (correct me if I am wrong) that I now have to design primer ...

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by ChIP
Sun Aug 15, 2010 3:51 pm
 
Forum: Molecular Biology
Topic: Primer design in the promoter region
Replies: 10
Views: 11118

Biochemistry/ Proteins & Amino Acids

... are acetylated, i.e. CH3CO-NH-CHR1CONH-CHR2CONH-etc. These fish will have: a) the same H+ and CO2 portions of the Bohr effect as fish without the acetylation modification b) a higher H+ portion of the Bohr effect, but no change in the CO2 portion of the Bohr effect, compared to fish without the ...

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by SimplyMe
Tue Feb 23, 2010 8:32 am
 
Forum: General Discussion
Topic: Biochemistry/ Proteins & Amino Acids
Replies: 4
Views: 5627
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