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Acetic

Acetic

(Science: chemistry) Of a pertaining to vinegar; producing vinegar; producing vinegar; as, acetic fermentation.

Pertaining to, containing, or derived from, acetyl, as acetic ether, acetic acid. The latter is the acid to which the sour taste of vinegar is due.

Origin: L. Acetum = vinegar, fr. Acere to be sour.


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TAE Buffer preparation

Please how do I prepare 10 M tae buffer containing 2M tris, 0.5M edta, 17.4M acetic acid and adjusted to pH 8.0 with NaOH .

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by dunamis
Thu Feb 26, 2015 6:05 pm
 
Forum: Molecular Biology
Topic: TAE Buffer preparation
Replies: 0
Views: 301

Methylene Blue Solution use in staining variety of bacteria?

... 136.1) ------ 1.36 g Distilled water ----------------------------------- 100 ml Mix to dissolve and adjust pH to 4.2 using concentrated glacial acetic acid Methyl Green Solution (0.5%) Methyl green (ethyl violet free from Sigma) ---- 0.5 g 0.1M Sodium acetate buffer, pH4.2 ------------- 100 ...

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by scytek
Sun Feb 22, 2015 9:22 am
 
Forum: General Discussion
Topic: Methylene Blue Solution use in staining variety of bacteria?
Replies: 0
Views: 272

Carnoy's fixation problem

... should be the original concentration of ethanol or methanol used in the preparation of Carnoy's fixative solution? I know it is alcohol: glacial acetic acid( 3:1). But what is the original concentration of the alcohol? Thank you very much :)

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by Sue4
Thu Apr 25, 2013 7:37 pm
 
Forum: Cell Biology
Topic: Carnoy's fixation problem
Replies: 0
Views: 1905

Problems making chemically competent cells

... Amount for 100ml RbCl (100mM) 1.2g MnCl2•4H2O (50mM) 0.998g Potassium acetate (30mM) 3ml (1M stock pH 7.5, 9.814g in 100ml solution, pH with acetic acid) CaCl2•2H2O (10mM) 0.15g Glycerol (15% w/v) 15g Final pH: 5.8 with dilute acetic acid. Don’t overshoot – if you do, start again RF2 Compound ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 3642

How does pH change during sporulation? - Help

Ah, yes! I managed to find a few articles in regards to acetate production (or I guess acetic acid). Which would make sense, cause then it could go into the TCA cycle (where rise in pH likely occured). Thanks for the response either way =).

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by keetner
Thu Oct 25, 2012 6:09 pm
 
Forum: Microbiology
Topic: How does pH change during sporulation? - Help
Replies: 2
Views: 2085
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