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Acetate

Acetate

(Science: biochemistry) a salt or ester of acetic acid, specifically, a synthetic textile fibre made from partially hydrolysed cellulose acetate, or a plastic-like film made from cellulose triacetate.

The terminal hydrogen atom in the molecule is replaced by a metal, for instance copper acetate, or where substitution is by a radical, for instance ethyl acetate.


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Methylene Blue Solution use in staining variety of bacteria?

... (toxic shock syndrome). Gram stain experiments are another type of a multi-step staining and de-staining experiment. Reagents 0.1M Sodium Acetate Buffer, pH4.2: Sodium acetate, trihydrate (MW 136.1) ------ 1.36 g Distilled water ----------------------------------- 100 ml Mix to dissolve ...

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by scytek
Sun Feb 22, 2015 9:22 am
 
Forum: General Discussion
Topic: Methylene Blue Solution use in staining variety of bacteria?
Replies: 0
Views: 271

Basic Knowledge about DNA Extraction

... be degraded with the addition of a protease. What’s more, the precipitation of a protein can be achieved by adding salt such as ammonium or sodium acetate. When the sample is vortexed with phenol-chloroform and centrifuged, the proteins will remain in the organic phase and can be drawn off and ...

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by 2444256703
Thu Jan 22, 2015 8:56 am
 
Forum: Genetics
Topic: Basic Knowledge about DNA Extraction
Replies: 0
Views: 754

Re: blank and substrate control give OD reads

... to harvest the enzyme 4) distribute substrate (pre-treated kenaf) equally to 96 deep well plate 5) add 250ul enzyme (from step 3) + 250ul sodium acetate into the substrate 6) incubate @ 50oC, 4hrs in waterbath 7) centrifuge to sediment the substrate 8) pipet 100ul reaction solution and transfer ...

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by intandiana
Sun Jan 19, 2014 5:57 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 6516

Re: blank and substrate control give OD reads

... and comparing against water at wavelengths near your OD measurement. Dear jonmoulton, thanks for your reply. >>>my buffer is sodium acetate pH 5.0. I'll compare it against water.

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by intandiana
Sat Jan 18, 2014 8:39 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 6516

blank and substrate control give OD reads

... variant than the wild type in hydrolysis kenaf. the reaction is done in 96 deep well plate by adding substrate (kenaf) + buffer (50mM sodium acetate, pH 5.0) + enzyme solution and incubate for 4 hours in waterbath. after 4hrs incubation, the reaction is centrifuged to collect only reaction ...

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by intandiana
Wed Jan 15, 2014 7:21 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 6516
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