Dictionary » A » Acetate

Acetate

Acetate

(Science: biochemistry) a salt or ester of acetic acid, specifically, a synthetic textile fibre made from partially hydrolysed cellulose acetate, or a plastic-like film made from cellulose triacetate.

The terminal hydrogen atom in the molecule is replaced by a metal, for instance copper acetate, or where substitution is by a radical, for instance ethyl acetate.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Re: blank and substrate control give OD reads

... to harvest the enzyme 4) distribute substrate (pre-treated kenaf) equally to 96 deep well plate 5) add 250ul enzyme (from step 3) + 250ul sodium acetate into the substrate 6) incubate @ 50oC, 4hrs in waterbath 7) centrifuge to sediment the substrate 8) pipet 100ul reaction solution and transfer ...

See entire post
by intandiana
Sun Jan 19, 2014 5:57 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3401

Re: blank and substrate control give OD reads

... and comparing against water at wavelengths near your OD measurement. Dear jonmoulton, thanks for your reply. >>>my buffer is sodium acetate pH 5.0. I'll compare it against water.

See entire post
by intandiana
Sat Jan 18, 2014 8:39 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3401

blank and substrate control give OD reads

... variant than the wild type in hydrolysis kenaf. the reaction is done in 96 deep well plate by adding substrate (kenaf) + buffer (50mM sodium acetate, pH 5.0) + enzyme solution and incubate for 4 hours in waterbath. after 4hrs incubation, the reaction is centrifuged to collect only reaction ...

See entire post
by intandiana
Wed Jan 15, 2014 7:21 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3401

Bio Research Paper Writing

... humans comparing cytokine production in CMV-negative and CMV-positive elderly and adult (Immune-nonhuman np). A compound, phorbol 12-myristate 13-acetate (PMA) was given to elderly and adults with CMV and without CMV; the collected data showed different CD8, CD4, and cytokine behavior between ...

See entire post
by NathanielZhu
Thu Apr 04, 2013 2:25 am
 
Forum: General Discussion
Topic: Bio Research Paper Writing
Replies: 1
Views: 12237

Problems making chemically competent cells

... nitrogen, then store at -70°C.) Solutions: RF1 Compound (end concentration) Amount for 100ml RbCl (100mM) 1.2g MnCl2•4H2O (50mM) 0.998g Potassium acetate (30mM) 3ml (1M stock pH 7.5, 9.814g in 100ml solution, pH with acetic acid) CaCl2•2H2O (10mM) 0.15g Glycerol (15% w/v) 15g Final pH: 5.8 with ...

See entire post
by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 2550
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 3,819 times. 
What links here | Related changes | Permanent link