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Absorptivity


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beers law concentration calculation

... I am asked to provide the concentration of a protein and I am given the number of Trp and Tyr residues, 3 and 6, so I have calculated the molar absorptivity as 3*5600+6*1420= 25320 M^-1cm^-1. Is this the correct units for this value? The path length is 1cm and the absorbance is 0.57 and the ...

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by protchem12
Thu Jan 12, 2012 5:24 pm
 
Forum: Molecular Biology
Topic: beers law concentration calculation
Replies: 8
Views: 5340

Re: Beer Lambert Law

So what happened to your exponent on the power of ten? I didn't check the extinction coefficient (now preferably called the molar absorptivity) of NADH. I'll take your word for it that it is 6.22E3 A/ M cm. What you haven't told anyone is what the volume of your sample of serum was and ...

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by blcr11
Thu Mar 05, 2009 2:42 pm
 
Forum: Molecular Biology
Topic: Beer Lambert Law
Replies: 1
Views: 6926

Re: beer-lambert law

I expect the units of the molar absorptivity to be M^-1 * cm^-1. This will give you an answer in molarity. You'll need to convert to micromoles. Watch out for the unit mol -- I interpret that as moles. Concentration is mol/l or M. You gave ...

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by jonmoulton
Fri Feb 27, 2009 4:53 pm
 
Forum: General Discussion
Topic: beer-lambert law
Replies: 4
Views: 7072

Re: beer lambert law

The stated coefficient is in the wrong units to be a molar absorptivity (or molar extinction coefficient). That would be in units of A per M (per 1 cm pathlength, but a 1 cm pathlength is usually assumed). If I understand what you said, a 1 mg/ml solution ...

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by blcr11
Tue Oct 07, 2008 4:48 pm
 
Forum: Molecular Biology
Topic: beer lambert law
Replies: 3
Views: 9353

spectrum of riboflavin

... at which these molecules absorb most strongly. Those wavelengths can be used to estimate concentrations of material in solution if you know the absorptivity at the particular wavelength. There are "standard" absorptivities assumed for DNA or protein (often called extinction coefficients)--see ...

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by blcr11
Sat Apr 14, 2007 4:01 pm
 
Forum: Cell Biology
Topic: spectrum of riboflavin
Replies: 5
Views: 6471
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