Absorptivity

So what happened to your exponent on the power of ten? I didn't check the extinction coefficient (now preferably called the molar absorptivity) of NADH. I'll take your word for it that it is 6.22E3 A/ M cm. What you haven't told anyone is what the volume of your sample of serum was and ...

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I expect the units of the molar absorptivity to be M^-1 * cm^-1. This will give you an answer in molarity. You'll need to convert to micromoles. Watch out for the unit mol -- I interpret that as moles. Concentration is mol/l or M. You gave ...

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The stated coefficient is in the wrong units to be a molar absorptivity (or molar extinction coefficient). That would be in units of A per M (per 1 cm pathlength, but a 1 cm pathlength is usually assumed). If I understand what you said, a 1 mg/ml solution ...

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... at which these molecules absorb most strongly. Those wavelengths can be used to estimate concentrations of material in solution if you know the absorptivity at the particular wavelength. There are "standard" absorptivities assumed for DNA or protein (often called extinction coefficients)--see ...

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can anyone help plz!! 1, Why are the units of absorptivity (a) m2 mol-1? 2, what are the units of the molar extinction coefficient (e) ? Thanks alot i really need some help

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