Absorbs

Yes, that sounds too low, at least for those wavelengths where methyl red absorbs strongly. Did you correct your concentrations for the dilution you made as you mixed your stock solution into liquid prior to making the spectrometer measurement?

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... case of 1:10 dilution) As you can see, the absorbance of oxaloacetate and malate in range 4 - 0.8 mM is rather low if any. On the other hand, NADH absorbs pretty well, thus if you were about to use these substances to track enzymatic activity you should go for with the NADH, because in the range ...

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the thing is, that in UV you measure everything that absorbs at 260 nm, including proteins, but mainly the nucleotides, which are in pretty high concentration in PCR reaction, right? So instead of purification of your product, you were purifying nucleotides ...

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Ok, but how Rhizobium Absorbs that nitrogen from that air gap?

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My lecturer taught me the molecular mechanism about how Rhizobium makes interaction with plants and how it converts N2 as Ammonia. My doubt is How it is done? Rhizobium is a soil bacteria and it is a anerobic. .It cause Root Nodules under the soil. . Then, How atmospheric nitrogen is absorbed for N2...

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