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A Band

The dark band of the muscle sarcomere that corresponds to the thick myosin (protein) filaments.The A band is situated on either side of the H zone of a muscle sarcomere, that is the area where contraction and relaxation of the muscle occurs, where sarcomeres overlap during muscle movements.


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Re: An interesting PCR smearing...I dont know what to do :(

... a similar problem. I am using 3 different primers to amplify 3 different exons of a gene . 1 of the exon is amplifying very well ( a single bright band ) but the other two exon amplification is not coming properly.and this has occured recently. ( even though the PCR reaction was standardized earlier ...

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by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 6236

Humans Natural Habitat/Optimal Environment

... you better suited to warmer temperatures, but that isn't to say that your interior metabolism would be happiest here. Though there is a narrow band of temperatures/humidities etc. that humans can survive in, within those limits there are a degree of flexibility. There is a reason you can sweat, ...

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by Babybel56
Tue Mar 04, 2014 6:04 am
 
Forum: Evolution
Topic: Humans Natural Habitat/Optimal Environment
Replies: 2
Views: 3500

plasmid extraction on 1.5% agarose gel

So I have two bands, the smaller that is around 8-9kb based on the marker ( my plasmids should be 8-9 kb), what is the upper band? Also I have three plasmids and I did this in a set of four replicates

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by cah2010
Wed Dec 18, 2013 2:23 am
 
Forum: Molecular Biology
Topic: plasmid extraction on 1.5% agarose gel
Replies: 5
Views: 1569

Weird - shaped band on western blotting

... My name is Laila and currently working with the hepatoma cells AH109A to see the cell cycle regulation protein. Recently I got a weird-shaped band on my western blot. It is like an upside-down of smiling face in each lane. I use the same loading buffer, electrophoresis buffer, lysis buffer, ...

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by namakulaila
Thu Oct 17, 2013 6:17 am
 
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 1308

Why does 28S rRNA appear as 4000 nt on a bioanalyzer

... quickly afterwards? (It's rRNA from HeLa cells, and I found an example on the internet showing Raji cells with a 28S/18S ratio of 2,7 and a 28S band size of 3900/4000 nt, so I figured the 28S rRNA would still be intact.) I can't find any explanation for this on the internet, so I hope someone ...

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by Nickythe100th
Sun Sep 08, 2013 9:05 am
 
Forum: Genetics
Topic: Why does 28S rRNA appear as 4000 nt on a bioanalyzer
Replies: 0
Views: 814
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