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Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging  

   

AUTHORS: 

  • Douglas B. Murphy

PRODUCT DETAILS:

  • Hardcover: 360 pages
  • Publisher: Wiley-Liss; 1st edition (December 15, 2001)
  • Language: English
  • ISBN-10: 047125391X
  • ISBN-13: 978-0471253914
  • Product Dimensions: 10 x 7.2 x 0.9 inches
  • Shipping Weight: 1.92 pounds
EDITORIAL REVIEWS

Book Description

Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope–skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image.

Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as:

  • Illuminators, filters, and isolation of specific wavelengths
  • Phase contrast and differential interference contrast
  • Properties of polarized light and polarization microscopy
  • Fluorescence and confocal laser scanning microscopy
  • Digital CCD microscopy and image processing

Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions.

An essential resource for both, experienced and novice microscopists. 

Book Info

Reviews such topics as illuminators, filters, and isolation of specific wavelengths; phase control and differential interference contrast; properties of polarized light and polarization microscopy; fluorescence and confocal laser scanning microscopy; and digital CCD microscopy and image processing. Includes practical demonstrations. For microscopists at any level. 
 

From the Back Cover

Fundamentals of Light Microscopy and Electronic Imaging
Douglas B. Murphy

Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope–skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image.

Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as:

  • Illuminators, filters, and isolation of specific wavelengths
  • Phase contrast and differential interference contrast
  • Properties of polarized light and polarization microscopy
  • Fluorescence and confocal laser scanning microscopy
  • Digital CCD microscopy and image processing

Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions.

Both experienced and novice microscopists will find Fundamentals of Light Microscopy and Electronic Imaging an essential resource.

From the Author(s)

The book retains a tested tradition of explaining image formation in the light microscope. Essential information is included about: aligning the microscope for different modes of light microscopy; the selection of filters, illuminators and fluorescent dyes; preparation and protection of cells for microscope observation, and other topics. Instructions are given on how to evaluate the qualities of a CCD camera and an electronic image, and how to operate and use electronic imaging systems.  

CUSTOMER REVIEWS

Best Introductory-Level Textbook, August 7, 2002

Dr. Murphy constructs a solid foundation on the basic concepts of geometrical optics, light, and color, and then provides excellent introductory reviews of important topics in light microscopy. The book is very well written and complex phenomena are clearly explained without the unnecessary math that often confuses students. Illustrations are numerous and help support the text very nicely, as do the suggested laboratory exercises that accompany each chapter. Discussions of digital cameras and image processing are timely and provide the essential concepts necessary to tackle more advanced treatises. In the opinion of the Molecular Expressions microscopy website team, this book is by far the best entry-level textbook in the field.

 


Rating: not rated | Added on: 23 Jan 2007

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