Difference between revisions of "Sticky end"

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For example, these two sticky ends can be joined together since they can form [[complementary]] [[base pair]]s in the [[overhang]] region:
 
For example, these two sticky ends can be joined together since they can form [[complementary]] [[base pair]]s in the [[overhang]] region:
  
5'-ATCTGACT           +   GATGCGTATGCT-3'
+
5'-ATCTGACT<font color="white"> ______</font>+ GATGCGTATGCT-3'
<br>3'-TAGACTGACTACG                 CATACGA-5'
+
<br>
 +
3'-TAGACTGACTACG<font color="white">________</font>CATACGA-5'
  
  

Latest revision as of 07:05, 2 October 2008

Definition

noun, plural: sticky ends

(molecular biology) A fragment of DNA (often produced by a staggered cut on the DNA using restriction enzymes) in which the terminal portion has a stretch of unpaired nucleotides, and the strands are not of the same length.


Supplement

The ends of the DNA are sticky because it can easily match up with a complementary overhang.

For example, these two sticky ends can be joined together since they can form complementary base pairs in the overhang region:

5'-ATCTGACT ______+ GATGCGTATGCT-3'
3'-TAGACTGACTACG________CATACGA-5'


Synonym: DNA end, cohesive end.
Compare: blunt end.
See also: restriction enzyme, overhang (molecular biology).

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