Maxam gilbert method

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Maxam Gilbert method

A method of dNA sequencing, based on the controlled degradation of a dNA fragment in a set of independent, nucleotide specific reactions. The resulting fragments have characteristic sizes depending on the sequence of the template, that can be resolved on a sequencing gel. Although no longer the main protocol, Maxam gilbert sequencing still has advantages, for example for oligonucleotides or covalently modified DNA.

See: dideoxy sequencing.

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