Why would a scientist want "stable overexpressing cells"?

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north3rner
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Why would a scientist want "stable overexpressing cells"?

Post by north3rner » Sun Apr 23, 2017 7:32 pm

Apparently it's a much used method in research, but I don't get it.

Here's an article that purportedly explains it.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296252/

In simple terms, what is the advantage of over expressing a certain gene to the point of creating a new phenotype? The latter (new phenotype) seems to define "over expressing genes".

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Vmedvil
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Post by Vmedvil » Tue Aug 15, 2017 10:23 am

Well, Over Expression is the act of adding or changing the promoter at some point that makes the RNA Polymerase generate more mRNA to the Ribosomes to be converted into proteins, This does a very different job than making a new phenotype, this regulates how many copies that this gene is sent to the Ribosomes per visit by a RNA Polymerase, a new phenotype would be changing the gene to do a slightly different function but sometimes you just want more of the protein generated, in these cases you would use Over-expression. Over-expression is much easier to do then make a new phenotype every-time that you wanted a different amount of the protein sent out, one code versus repeating the same code over and over again, just add a promoter that simple, in many ways you are making a new phenotype by over-expression, just one with a amount of mRNA sent out that you control, rather than actually changing the protein structure itself by changing the coding region.
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