Search found 3 matches

by stephroche
Fri Apr 29, 2011 2:01 pm
Forum: Molecular Biology
Topic: Molecular biology master mix preparation question?
Replies: 2
Views: 2377

Buffer 10µL
Primers : 1µL each
dNTP : 1µL
Taq Pol : 0.25µL
H2O : 34.75µL

+ 2µL DNA
by stephroche
Fri Apr 29, 2011 1:57 pm
Forum: Cell Biology
Topic: Do we know all the proteins (and their primary structure!)?
Replies: 7
Views: 12037

We do not know every protein sequence. The genes do not code to the post traductionnal modifications. As exemple, on the collagen sequence, we have a repeat of proline but in the real molecule, some aa are hydroxyproline. In addition, some protein need to loose a peptide to be active or to modify th...
by stephroche
Thu Apr 28, 2011 12:55 pm
Forum: Cell Biology
Topic: Why do we need to eat so much protein? A.a. can be reused
Replies: 5
Views: 6552

We have essential and not essential amino-acids. The essential AA are not produced by our body and need to be found in our food. For not essential AA, some are not enough produced by our cells. Among all these needed AA, some are produced by plant cell and some by animals. Then, we need to eat both ...