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- Tue Jan 24, 2017 9:47 am
- Forum: Cell Biology
- Topic: Why cells dies in flow cytometry analysis??
- Replies: 2
- Views: 6929
Recently, I used FACS-buffer (PBS, 2 mM EDTA, 25 mM HEPES) for antibody dilution and washing. Although, clump formation was less but it was present. Besides, I would also like to know that do the presence of HEPES buffer and EDTA interfere with the result while making antibody dilution buffer.
Agree with Fornita, bacterial contamination is easily detected by visual inspection of the culture within a few days of it becoming infected. Infected cultures usually appear cloudy (i.e., turbid), sometimes with a thin film on the surface. Under a low-power microscope, the bacteria appear as tiny, ...
Hi, I think it depends on the plate you want to use. For most ELISA plates, you simply add your protein of interest (here your ab) in buffer to the plate and incubate, e.g. over night at 4°C or for 2h at RT. But as I wrote, it depends a lot on your plate and your protein. Sometimes pre-coating is ne...