Search found 10 matches

by chance
Mon Nov 16, 2015 2:06 am
Forum: Cell Biology
Topic: Do we know all the proteins (and their primary structure!)?
Replies: 7
Views: 11136

Re: Do we know all the proteins (and their primary structure

Firstly, I think it is impossible that we have known all the proteins structure. Polypeptides are chains of amino acids. Proteins are made up of one or more polypeptide molecules.The amino acids are linked covalently by peptide bonds. The graphic on the right shows how three amino acids are linked b...
by chance
Tue Nov 10, 2015 5:59 am
Forum: Molecular Biology
Topic: Method for enhancing solubility of recombinant protein
Replies: 0
Views: 3416

Method for enhancing solubility of recombinant protein

The E. coli expression system is extensively used as the first choice in genetic engineering due to its high productivity, high growth and production rate, ease of use, and economy, etc. However when expressed in e.coli, most recombinant proteins deposit in the form of insoluble and inactive inclusi...
by chance
Tue Nov 03, 2015 2:08 am
Forum: Cell Biology
Topic: CHOICE OF MAMMALIAN CELL HOST
Replies: 1
Views: 5738

CHOICE OF MAMMALIAN CELL HOST

Although a variety of mammalian cell hosts are available for protein production, only a small number have emerged as systems of choice for production of proteins to be used clinically.The narrowing down of choices is largely due to the need for cell lines that: (1) are capable of continuous growth; ...
by chance
Thu Oct 15, 2015 9:13 am
Forum: Molecular Biology
Topic: Ni-NTA-Columns
Replies: 4
Views: 7265

Re: Ni-NTA-Columns

1. Imidazole concentration is too high. When eluting with imidazole, each gradient must elute completely; 2. The cleaning of sample is not sufficient, unable to remove unbound contaminants. You can extend the cleaning time and increase the imidazole concentration to improve the cleaning effect; 3. I...
by chance
Thu Oct 15, 2015 9:04 am
Forum: Molecular Biology
Topic: experiences with ni-affinity protein purification??
Replies: 4
Views: 11991

Re: experiences with ni-affinity protein purification??

1、the high concentration of imidazole can change the pH of your buffer. 2、It may be the toxic effect of nickel ions and you can use a non-toxic Ni column. when protein is eluted, nickel ions is still remained in solution to maintain recombinant protein production activity to be best to look at the d...
by chance
Wed Oct 14, 2015 10:04 am
Forum: General Discussion
Topic: how to decrease the degradation of protein in purificaton
Replies: 4
Views: 4413

Re: how to decrease the degradation of protein in purificato

We commonly use protease inhibitors, such as PMSF (0.2mM), Leupeptin (1ug/ml), EDTA (1mM), A Pepstatin (1ug/ml), Aprotinin (1ug.ml). The above are some protease inhibitors and dosage used in recombinant protein purification experiments. You can mix with several protease inhibitors in the experiment,...
by chance
Fri Sep 11, 2015 7:08 am
Forum: Cell Biology
Topic: Cancer more than animals animals
Replies: 9
Views: 16812

In the plant kingdom,like human being, plant also suffer from cancer. Plants from embryonic development, growth and flowering, its whole life process are controlled by genes within the cell, the various tissue cells in different gene under the control of a methodical metabolism. But when plants were...
by chance
Thu Sep 10, 2015 8:34 am
Forum: Molecular Biology
Topic: Cancer Cells
Replies: 2
Views: 8003

Cancer cells from cancer related genes, the cancer related gene transcription into cancer cells.
by chance
Wed Sep 09, 2015 5:59 am
Forum: Molecular Biology
Topic: translation from DNA
Replies: 5
Views: 16526

Because there is no the Enzyme to directiy translate the DNA into proteins.
http://www.biologicscorp.com/
by chance
Wed Sep 09, 2015 5:35 am
Forum: Cell Biology
Topic: Totipotent Stem Cell From Molecular Components
Replies: 3
Views: 15916

PCR is an in vitro DNA amplification technology, which is dependent on the DNA polymerase chain reaction, which is the three step reaction of DNA polymerase chain reaction, which makes the DNA fragment increase in number and the number of specific gene fragments in short time. The basic principle of...