Search found 10 matches

by poloke
Sat Aug 11, 2012 2:29 pm
Forum: Microbiology
Topic: question on genotype
Replies: 1
Views: 5447

question on genotype

Dear all, Anyone here who can tell me what this means (part of a genotype of a yeast): his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 I have always been told that Δ before a gene, means that the gene is gone (knock out mutant). But what does it mean if it stands at the end (his3Δ) or as in my other example: his3Δ1/his...
by poloke
Sat Aug 11, 2012 2:00 pm
Forum: Bioinformatics
Topic: searching for gene sequences, automated
Replies: 8
Views: 8288

Re:

Even the BLAST _at_ NCBI web page gives you the opportunity to search with several sequences at once (I'm not aware what's the limit, maybe 20 or 200 sequences;) I seem to have overlooked this, where can you find this? Can I simple add multiple sequences then? How do I seperate them when inserting ...
by poloke
Sat Jul 14, 2012 11:53 am
Forum: Bioinformatics
Topic: searching for gene sequences, automated
Replies: 8
Views: 8288

Re: searching for gene sequences, automated

As a student, I do not have thousands of dollars to spend :p
by poloke
Thu Jul 12, 2012 11:27 am
Forum: Bioinformatics
Topic: searching for gene sequences, automated
Replies: 8
Views: 8288

Re: searching for gene sequences, automated

Do you have a specific link for it? I can go to the bookshelf, but those are just books, isnt there an online tutorial or something like that?
Or perhaps you know some programs that are easy to use?
by poloke
Wed Jul 11, 2012 12:58 pm
Forum: Bioinformatics
Topic: searching for gene sequences, automated
Replies: 8
Views: 8288

searching for gene sequences, automated

Hallo all, Recently I sequenced 3 pieces of DNA , now its pretty easy to check for similar genes in the ncbi databank: you just enter your sequence and you search... and you repeat this 3 times, since I had 3 genes.. However , what if someone has for example 300 genes to check? Is their any option t...
by poloke
Mon Jun 25, 2012 11:39 am
Forum: Bioinformatics
Topic: question on sequencing
Replies: 5
Views: 7087

Re:

The number of fragment of each size depends on probability. The shorter ones are the most common. There is one fluorophore per fragment. So the the very numerous short fragment are very bright, to the point that they can be hard to read correctly. Ah yes, I see what you mean! Thanks a lot. This is ...
by poloke
Thu Jun 21, 2012 9:08 pm
Forum: Bioinformatics
Topic: question on sequencing
Replies: 5
Views: 7087

Re:

The first fragments usually have large overlapping peaks because there are a lot of the short fragments. That means very strong (saturating and leeching light beyond its location) signal and wide, less defined band. So you that if the fragments where longer, the fluorescent label would not be such ...
by poloke
Mon Jun 18, 2012 8:46 pm
Forum: Bioinformatics
Topic: question on sequencing
Replies: 5
Views: 7087

question on sequencing

I am learning about sanger sequencing and I do not understand the following (from wiki): "Common challenges of DNA sequencing include poor quality in the first 15–40 bases of the sequence and deteriorating quality of sequencing traces after 700–900 bases" How come the first bases are bad? ...
by poloke
Sat Jun 02, 2012 3:13 pm
Forum: Microbiology
Topic: Question about phage library
Replies: 3
Views: 5640

Re:

no, it's physical link between DNA sequences or physical link between encoded proteins and not physical link between DNA and protein, i.e. the DNA is connected and expressed as only one gene and thus there is only one protein composed of the capsid protein and your protein of interest. Ok, that doe...
by poloke
Sat Jun 02, 2012 2:06 pm
Forum: Microbiology
Topic: Question about phage library
Replies: 3
Views: 5640

Question about phage library

Dear all, I was reading about phage library and stumbled on this website (m). Now, I understand how phage library works, but there is something in the explenation I do not get. Its about this part, and more specific the part in bold. "Technical description of phage display The starting point is...