mannose extraction with methanol/chloroform

[spinkster]

Hi,

ive been looking in my biochem books for why mannose can be extracted with methanol/chloroform in glycoprotein synthesis.

I dont really get it, does the methanol/chloroform attach to a dolicol which is attached to two phosphate groups which is then attached to mannose?

any help on this would be great (as i am probably not understanding it correctly)

cheers

Matt

[MrMistery]

yes,i think you have it exactly on target. Glycoprotein synthesis occurs by attaching sugars evolved from dolichol phosphate. Dolichol, of course, is a lipid, and can be extracted by using methanol/chloroform.

[spinkster]

Also do you know if dolicol has any other roles in the cell.
I know it has another function as a GPI anchor but is anyone aware of any others?

cheers