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microscopic measurements...

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microscopic measurements...

Postby pinkypig » Sat Mar 15, 2008 12:14 pm


Hi can anyone help me with this....basically i want to know that if you want to measure an object under a microscope and the eye piece is smaller than the object even at low power how would you accuratly measure the object?

Thanks.
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Re: microscopic measurements...

Postby pinkypig » Sat Mar 15, 2008 12:22 pm

and also...is it better to use a low power or high power magnification when measuring a cell?and why is that the case?
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Postby MichaelXY » Mon Mar 17, 2008 5:46 am

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Postby pinkypig » Mon Mar 17, 2008 12:56 pm

Thanks for the above site it is very useful!
but i'm still not sure about the first question asked which was:

i wanted to know that if you want to measure an object under a microscope and the eye piece is smaller than the object even at low power how would you accuratly measure the object?

Thanks
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Re: microscopic measurements...

Postby MichaelXY » Mon Mar 17, 2008 5:58 pm

Well, if I understand you correctly. You want to measure an object that exceed the FOV of your lowest power. My first thought is that if the object is large enough, just use a ruler. But let's say it is not. I made a image to illustrate.
So say you computed a FOV for your low power setting to be 4500um. Look at the picture shown. In this example. We can approximate 2.25 x 4500um.
Hope that helps.

Image
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Postby pinkypig » Mon Mar 17, 2008 8:41 pm

what does FOV mean?
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Postby MrMistery » Mon Mar 17, 2008 8:47 pm

field of view
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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Postby pinkypig » Mon Mar 17, 2008 9:29 pm

So would you have to move the slide across to measure the whole object?
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Re: microscopic measurements...

Postby MichaelXY » Mon Mar 17, 2008 11:40 pm

Higher quality microscopes have an adjusting knob for moving the stage, which is essentialy moving the slide, but with more precision. It is mentioned in that link I provided earlier, as well as FOV.
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Postby pinkypig » Tue Mar 18, 2008 11:12 am

oh rite, thanks alot!
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