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Staining Bacteria...

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Staining Bacteria...

Postby ImDaSTyle » Tue Feb 05, 2008 1:46 am

I can't seem to find the answers anywhere..not even online or on the book...please help =(

Why aren't the magnifications of both ocular lenses of a binocular microscope used to calculate total magnification?

What is the total magnification for each lens setting on a microoscope with 15x oculars and 4x, 10x, 45x and 97x objectives lens?

What is the consequence of leaving a stain on the bacterial smear too long (overstaining)?

What is the consequence of not leaving the stain on the smear ong enough (understaining)?

Thanks :3
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Postby Cat » Sun Feb 17, 2008 12:30 am

You should be able to find this in the book!!!
Q1: binocular is for looking with two eyes, if you look with just one, total magnification does not change.
Q2: total mag = ocular mag x objective mag.
Q3 and 4: Which stain are you talking about???
King Cobra
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Postby EmmVeePee » Wed Feb 20, 2008 6:29 pm

Leaving a stain on too long generally stains everything; leaving little contrast.
Leaving a stain on not long enough doesn't stain enough; leaving little contrast.
Undergraduate, Microbiology
Pennsylvania State University
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