Login

Join for Free!
112378 members


Staining Bacteria...

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderator: BioTeam

Staining Bacteria...

Postby ImDaSTyle » Tue Feb 05, 2008 1:46 am

I can't seem to find the answers anywhere..not even online or on the book...please help =(

Why aren't the magnifications of both ocular lenses of a binocular microscope used to calculate total magnification?

What is the total magnification for each lens setting on a microoscope with 15x oculars and 4x, 10x, 45x and 97x objectives lens?

What is the consequence of leaving a stain on the bacterial smear too long (overstaining)?

What is the consequence of not leaving the stain on the smear ong enough (understaining)?

Thanks :3
ImDaSTyle
Garter
Garter
 
Posts: 1
Joined: Tue Feb 05, 2008 1:43 am

Postby Cat » Sun Feb 17, 2008 12:30 am

You should be able to find this in the book!!!
Q1: binocular is for looking with two eyes, if you look with just one, total magnification does not change.
Q2: total mag = ocular mag x objective mag.
Q3 and 4: Which stain are you talking about???
Cat
King Cobra
King Cobra
 
Posts: 625
Joined: Thu Feb 14, 2008 7:40 pm

Postby EmmVeePee » Wed Feb 20, 2008 6:29 pm

Leaving a stain on too long generally stains everything; leaving little contrast.
Leaving a stain on not long enough doesn't stain enough; leaving little contrast.
Greg
Undergraduate, Microbiology
Pennsylvania State University
User avatar
EmmVeePee
Coral
Coral
 
Posts: 186
Joined: Thu Apr 14, 2005 2:52 am
Location: Philadelphia Area, PA



Return to Microbiology

Who is online

Users browsing this forum: No registered users and 2 guests

cron