Login

Join for Free!
17836 members
Home Forum Dictionary Articles Tutorials Books Directory Share your work

SDS

Genetics as it applies to evolution, molecular biology, and medical aspects.

Moderator: BioTeam

Post a reply

SDS

Postby okmedcui on Sun Jan 27, 2008 3:08 pm

Hi,
During the experiment of extraction of genomic DNA from E.coli, we added 2% SDS/2N NaOH solution to a cell suspension containing lysozyme. What's the use of SDS?
thx
okmedcui
Garter
Garter
 
Posts: 3
Joined: Sun Jan 27, 2008 1:59 pm
Top

Postby mith on Sun Jan 27, 2008 4:10 pm

look it up
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
User avatar
mith
Inland Taipan
Inland Taipan
 
Posts: 4642
Joined: Thu Jan 20, 2005 8:14 pm
Location: Berkeley, CA
Top

Postby okmedcui on Sun Jan 27, 2008 4:20 pm

I understand that it denatures proteins.
but bacteria were lysed by lysozyme and lysis buffer, why need sds?
okmedcui
Garter
Garter
 
Posts: 3
Joined: Sun Jan 27, 2008 1:59 pm
Top

Postby mith on Sun Jan 27, 2008 4:53 pm

http://www.bio.davidson.edu/COURSES/GEN ... SPAGE.html
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
User avatar
mith
Inland Taipan
Inland Taipan
 
Posts: 4642
Joined: Thu Jan 20, 2005 8:14 pm
Location: Berkeley, CA
Top

Postby okmedcui on Mon Jan 28, 2008 9:45 am

Thank u!
okmedcui
Garter
Garter
 
Posts: 3
Joined: Sun Jan 27, 2008 1:59 pm
Top
Post a reply

Return to Genetics

Who is online

Users browsing this forum: No registered users and 0 guests