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Isoelectric Point

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Isoelectric Point

Postby rikajaj on Thu Nov 29, 2007 2:12 am

Can someone tell me how to find the theoretical isoelectric point of a given protein? Is there an equation? Or a search tool? I tried pubmed but I couldn't find it for the protein I need to do a paper on.

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Postby mith on Thu Nov 29, 2007 2:57 am

http://www.expasy.ch/tools/pi_tool.html

this is a predictor....
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Postby MrMistery on Thu Nov 29, 2007 8:06 pm

I am no expert, but an isoelectric point determination program should work with PDB files, as you need to know its 3D shape. It doesn't matter so much what aminoacids are in the protein, but rather what aminoacids are on the surface...
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Postby woolleyy on Fri Nov 30, 2007 2:12 am

As I understood it a Pi detector was pretty shabby, as nothing can really substitute for hard evidence from a little experimenting...
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Postby MrMistery on Sat Dec 01, 2007 6:44 pm

I would think a program that would work with PDB files should get them pretty close, as it could take into account all the aminoacids on the surface. Sure, nothing beats a good isoelectric focusing, but it is good for a start.
However, i don't know if such a program exists...
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