Biology-Online • View topic - NEED HELP!

Join for Free!
122352 members


Everything on bioinformatics, the science of information technology as applied to biological research.

Moderator: BioTeam

Postby h2so4hurts » Mon Nov 26, 2007 5:01 pm

This sounds like a homework question. I'm pretty sure there are easy tests to determine which major operons regulate your gene... Its probably in your book. Changing the mechanism is pretty easy, just change the operator sequence upstream of the gene. I'm not really sure what the limitations of your answer are though, but there are plenty of solutions.
User avatar
Posts: 32
Joined: Fri Nov 16, 2007 7:13 pm
Location: Iowa City, IA

Postby mith » Mon Nov 26, 2007 5:20 pm

Microarrays! (That's my answer for everything but it works)
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
User avatar
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN

Postby canalon » Tue Nov 27, 2007 3:09 am

Well if you are in E. coli K12(aka MG1655) or O157:H7 you can lookup the genome. Other wise I suggest:
- Make a probe with your gene
- Southern on PFGE profile or BAC Library
- Sequence clone or cloned PFGE fragment

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Return to Bioinformatics

Who is online

Users browsing this forum: No registered users and 0 guests