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glass vs. plastic when working with lipidsModerator: BioTeam
4 posts • Page 1 of 1
glass vs. plastic when working with lipidsHi,
I need to analyze the fat content of stool samples from mice. I found a protocol that involves chloroform:methanol extraction and weighing the extracted fat. The entire protocol is with glass tubes, but we don't have a centrifuge that fits glass tubes. How important is it to use glass and not plastic? I anyway can't use glass, so is it worth bothering with this protocol? Thanks!
i don't see how glass/plastic tubes would make a significant difference. But my lab experience is extremely limited.
"I have no intention of stopping anytime soon. I want to understand the universe and answer the big questions, that is what keeps me going" - Stephen Hawking
they do stick to plastic, but if they are in a methanol chloroform solution i doubt they do
"I have no intention of stopping anytime soon. I want to understand the universe and answer the big questions, that is what keeps me going" - Stephen Hawking
4 posts • Page 1 of 1
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