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centrifugation

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centrifugation

Postby gumshoes_15 on Mon Nov 12, 2007 10:25 pm

What are the disadvantages and advantages of using centrifugation to extract lipids?
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Postby Promethean on Fri Nov 23, 2007 4:03 am

Can you expand on that? A little more detail on what you are trying do would be helpfull?
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Postby MrMistery on Fri Nov 23, 2007 12:55 pm

i've never heard of extracting lipids with a centrifuge. usually it's done with a solvent that disolves lipids but not anything hydrophilic. I don't see why you would bother using a centrifuge.
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Postby biohazard on Sat Nov 24, 2007 9:04 pm

Maybe this is about separating cell organelles (including any lipid-containing ones) with a gradient (ultra)centrifugation? At least that method is fairly straightforward to do if you have the equipment. I'm not sure either if I've heard of any other lipid separation protocols using centrifuges :P
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