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plasmid DNA mwt calculations

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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plasmid DNA mwt calculations

Postby yammie » Mon Jan 24, 2005 10:23 am

hi
I have some basic doubts in calculating the charge ratio between polyethylenimine(PEI) and plasmid DNA.PEI=25k,PDNA=6.4 kb.How to calculate the N/P ratio(nitrogen of PEI/phosphate of DNA).Kindly help.Most of them say as 1:4(DNA:PEI) wt/wt .What does this mean?/how to work it out?
yammie
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