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How to isolate protein extract from mice adipose tissue

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How to isolate protein extract from mice adipose tissue

Postby mrna810 » Tue Jun 07, 2005 3:40 pm

Thanks
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SDS page

Postby mothorc » Wed Jun 08, 2005 1:19 pm

use SDS Page but first, denature protein by SDS (it just make protein become negative charge),
second, separate protein and glycerit by centifuge
then separate on SDS gel
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but I mean how to prepare protein extracts from tissue?

Postby mrna810 » Thu Jun 09, 2005 2:35 pm

TRIzol kit? or Tri Reagent?
Anyone has experience in getting proteins from animal tissue?
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