Discussion of all aspects of cellular structure, physiology and communication.
Methylene blue has a few risks; you'll just have to research it
Also, the haemocytometer has already been mentioned as an alternative several times, if you bothered to read the thread at all.
Hi everyone, I am new to these forums. I have to do this plan. I am really confused actually, although it seems that it isn't the hardest.
For the predicition, do u really need to talk about the high temp? as it is only asking about the lowest temp. I am gonna draw graph of rate of activiy/temp ?
What are the temp everyone gonna use?
Regarding the amount of the glucose and the yeast, how much of each do u need?
Any help would be appreciated.
Oh, btw at low the temp, the enzyme of the yeast don't die, they are just not active due to the low kinetic enery of the particles, right?
Does anybody know which variables i should keep the same in this experiment? I've currently thought of;
But i dont really understand why i'm keeping the time the same?
I'd be grateful for any help!! Thanks
hey, i think i kinda understand what to do for this, but can sum1 jus help me with a few points please? 2 days left...
1) Is methylene blue blue when yeast is alive and clear when it's dead?
2) finding the temperature - do i heat the yeast and solution separately to the temp i want, then pour them into 1 tube and add meth blue?
3) safety precautions - i've sed to wear goggles, wash hands and not to consume the stuff. is there anything else?
the methylene blue is an indicator
therefore needs to be added to the solutions AFTER placing them under certain tempratures too see wheather it turns clear (enzymes have not been denatured) or remains blue (have been denatured) when looking under the microscope to check.
reducing tempratures wouldn't denature the enzymes but would slow the reaction down somewhat.
i was wondering how to go about finding the lowest temprature that denatures the enzyme?
I think u answered your own question?
Oh and just a bit more friendly advice which no one has mentioned..... our teacher has kind of hinted that you need to add a buffer solution to make sure the ph stays the same in all test tubes... hope this helps people
Oh and ps happy mayday everyone
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