Login

Join for Free!
118841 members


Imagination about electrophoresis

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Imagination about electrophoresis

Postby Tae Jun, Yoon » Fri Apr 13, 2007 11:47 am

I am undergraduate and I learned about electrophoresis recently. It was
interesting. So I tried to imagine the practical application of electrophoresis. I want other great ideas from you. Please give me a reply!! :D
First, It can be used to distinguish prion protein and prion protein scrapie which can cause BSE. Nowadays, many countries think the beef from America can cause BSE. To prevent the disease, we should distinguish the meat. So I think it is useful to distinguish bad beef by electrophoresis.
Second, it can be applied to find out cancer. Different from many kinds
of cells, cancer has an enzyme called telomerase, which can restore the telomere : Well, of course other cells also have the telomerase : bortany cells, marrow cell and so on. But most cells in our body has no telomerase as far as I know.... So I think the electrophoresis can be a means for distinguish ordinary cells from cancer.
Third, it can be a means for bioinformatics and protemoics. Especially, many bioinstitutes demand database about protein or gene. So I think the electrophoresis can be applied to research..........

Do you have other ideas or Could you tell me if there's wrong idea in this writing?
User avatar
Tae Jun, Yoon
Garter
Garter
 
Posts: 36
Joined: Fri Mar 23, 2007 11:22 am
Location: Seoul, Korea

Re: Imagination about electrophoresis

Postby kotoreru » Fri Apr 13, 2007 5:42 pm

Tae Jun, Yoon wrote: But most cells in our body has no telomerase as far as I know....


I'm not a molecular biologist, but I'm fairly sure that telomerase is quite ubiquitous in our cells - without it surely our cell divisions would soon reduce our chromosomes' length unacceptably.

Am I wrong?
User avatar
kotoreru
Coral
Coral
 
Posts: 400
Joined: Thu Apr 12, 2007 9:25 pm
Location: London

Postby canalon » Fri Apr 13, 2007 8:53 pm

First, prp and prp* have the same amino acid sequence, so protein electrophoresis, which is often done in denturing conditions is not the best tool to detect the presence of prp* in beef. In fact antibodies are better suited since they are sensitive to conformation.

Second, there are plenty of cancer markers, the problem is usually not to determine if a cell is cancerous or not, it's to locate cancerous cells before the cancer completely invade the body.

Third, well it is called proteomics. It relies on 2D gel electrophoresis and is a widely available technique. Interpretation of data, due to the fact that protein sequencing is still not easy is the tricky part. And electrophoresis of nucleic acids is well... even more common. I have the result of 600 PCR under my eyes in the form of a picture of a DNA electrophoresis and I am scoring them. Tedious, but the common work of many molecular biologists...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada


Thanks for catching up my mistakes!!

Postby Tae Jun, Yoon » Sat Apr 14, 2007 9:45 am

Thanks for catching up my mistakes!!

But one question, then what is the difference between PrP and PrP* in the structure?
User avatar
Tae Jun, Yoon
Garter
Garter
 
Posts: 36
Joined: Fri Mar 23, 2007 11:22 am
Location: Seoul, Korea

Postby canalon » Sat Apr 14, 2007 4:13 pm

I do not have aclue of the exact nature of structure differenec. Besides even if I knew, i do not think it would be easy to describe in word. I suggest you search internet (swissprot?) for structure of the 2 forms of the protein if you are really interested.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Postby lara » Sun Apr 15, 2007 1:16 am

the prion proteins have changed conformation-more beta pleats than the alpha helices and the conformation of normal proteins are changed by these abnormal proteins.
lara
Coral
Coral
 
Posts: 131
Joined: Sun Sep 25, 2005 5:10 am

Postby Jammerz » Mon Apr 16, 2007 4:20 am

Yes, the increased beta-pleat structure is the main structural difference between the two conformations like lara said.
Jammerz
Death Adder
Death Adder
 
Posts: 77
Joined: Thu Mar 22, 2007 4:02 am
Location: USA

Postby mounirbio » Mon Apr 23, 2007 5:31 pm

hello how are u jaen tun
i am sanae and i am undergraduate in morocco
*u must make migration of sain and disease protein by electrophorisis, and after u show the the weight molecular of eache protien.
mounirbio
Garter
Garter
 
Posts: 5
Joined: Thu Apr 19, 2007 2:33 pm


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 3 guests