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Antiserra+blood product's chemistry

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Antiserra+blood product's chemistry

Postby Abu Anzar » Sun Apr 08, 2007 2:20 pm

I have an assignment in which I have to study the chemistry of product resulted after reacting blood with antiserra A and B,

I know the chemistry of blood and can get chemistry of Antiserra

but the question remain how to study the chemistry of product

In Lab we are furnished with hemoanalyzer, and spectrometer any hint? to what I go and how can I study it, experimentally
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Postby Abu Anzar » Sun Apr 08, 2007 3:00 pm

I think I need to clearify what I am trying to say

Study of blood after reacting it with antiserra


i.e, study of burst particles :P
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Postby Dr.Stein » Sun Apr 08, 2007 4:04 pm

The principle of bloods against their antisera is antigen:antibody. You can figure it out, ok? ;)
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Postby Abu Anzar » Sun Apr 08, 2007 4:11 pm

I know that the principle of blood is against that of antiserra, the headache is not of dealing antiserra's principle or its chemistry the headache is how to study the chemistry of resultant ( when blood reacts with antiserra and give some result )

let

b=blood
a=antiserra

b+a=c (product/resultant)

I want to study the chemistry of this 'c'
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Postby Darby » Mon Apr 09, 2007 12:41 am

The problem with what you're asking is that the antibody-antigen complex winds up holding red blood cells together, so it's not a simple chemical complex.
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Postby Dr.Stein » Mon Apr 09, 2007 3:32 am

Darby is correct. Actually Abu does NOT know anything about the principle as he said, if he understands, he wouldn't answer that way. Hemagglutination is not something like CO2 + H2O ---> H2CO3. It's immunological process and by understanding the principle we will get the answer ;)
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Postby Abu Anzar » Mon May 07, 2007 6:47 pm

late replying

I know about that principle and theoritically I know how to study it
but I was interested in using any technique or device to read it out, because ma task was to implement any logic for studying its chemistry with the help of digital circuits

might b there is something wrong in the way of questioning.
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Postby david23 » Mon May 07, 2007 7:06 pm

do you just want to study the chemistry of the antigen antibody complexes, or the cells. ooor. I say lets do a simple western blot to assay the complexes, and use flow cytometry to study the actual blood cells. Someone else can probably offer a better solution. I actually dont know what you are trying to do, so this is the best help I can give.
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Postby Abu Anzar » Mon May 07, 2007 7:16 pm

yah!
I know I am not able to deliver my question properly that is making problem :(
let
close the chapter for now :(
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Postby david23 » Mon May 07, 2007 7:29 pm

hey just private message me if you need more stuff
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