Login

Join for Free!
112030 members


Agar plates and E. coli... Simple question...

Debate and discussion of any biological questions not pertaining to a particular topic.

Moderator: BioTeam

Agar plates and E. coli... Simple question...

Postby FlashBulb » Wed Jan 19, 2005 9:10 pm

Hello everyone!

I'm trying to construct a biology experiment, and I was wondering if it is correct and proper to take a sample of baterial growth from one agar plate and place the sample on a new fresh agar plate?

Any help would be GREATLY appreciated! :)

Thanks,

--Dave
User avatar
FlashBulb
Garter
Garter
 
Posts: 5
Joined: Wed Jan 19, 2005 9:08 pm

Postby ERS » Wed Jan 19, 2005 9:30 pm

In my experience, and others may vary and be more correct, making a new streak from plate to plate sometimes goes better if you grab a colony from plate 1and culture it in a test tube with fresh media, then use that to streak plate 2. The only downside to this is that it adds an extra step= extra time... so it may not be feasible depending on your circumstances.

anyone else with thoughts?
ERS
ERS
Inland Taipan
Inland Taipan
 
Posts: 137
Joined: Thu Dec 16, 2004 5:13 am

Postby FlashBulb » Wed Jan 19, 2005 10:26 pm

ERS wrote:In my experience, and others may vary and be more correct, making a new streak from plate to plate sometimes goes better if you grab a colony from plate 1and culture it in a test tube with fresh media, then use that to streak plate 2. The only downside to this is that it adds an extra step= extra time... so it may not be feasible depending on your circumstances.

anyone else with thoughts?
ERS


I appreciate your help :wink:

I have one question about your post: Should I scrape a cultured agar plate and place the sample into a proper liquid medium, and finally, after letting the liquid culture sit for a while, take a sample for that liquid culture and place it on a fresh agar plate? :roll:

I'm kinda a new timer to these type of experiments, and I hope I'm not bugging anyone with my petty questions :P :oops:
User avatar
FlashBulb
Garter
Garter
 
Posts: 5
Joined: Wed Jan 19, 2005 9:08 pm


Postby FlashBulb » Thu Jan 20, 2005 9:35 pm

Can anyone answer the quick follow-up question I had? ^^^

Thanks :wink:
User avatar
FlashBulb
Garter
Garter
 
Posts: 5
Joined: Wed Jan 19, 2005 9:08 pm

Postby ERS » Thu Jan 20, 2005 11:04 pm

no worries we like all questions! BTW, sorry about the delay here with your second question. What you described should work quite fine. Make certain that you are using good technique so you don't contaminate during the various transfers.

Good luck!
ERS
ERS
Inland Taipan
Inland Taipan
 
Posts: 137
Joined: Thu Dec 16, 2004 5:13 am

Postby FlashBulb » Thu Jan 20, 2005 11:41 pm

ERS wrote:no worries we like all questions! BTW, sorry about the delay here with your second question. What you described should work quite fine. Make certain that you are using good technique so you don't contaminate during the various transfers.

Good luck!
ERS


Thank you VERY much for all of your help. I really greatly appreciate it :wink:

--Dave
User avatar
FlashBulb
Garter
Garter
 
Posts: 5
Joined: Wed Jan 19, 2005 9:08 pm


Return to General Discussion

Who is online

Users browsing this forum: No registered users and 6 guests

cron