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DNA electrophoresis

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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DNA electrophoresis

Postby 2810712 » Mon Jan 24, 2005 5:24 am

I have seen marks denoting the number of nucleotides along the gel which actually denote the molecular weight. But , do the polynucleotides with same number of nucleotides have same molecular wt.always? I think , they would not , as the proportions of A ,T ,G & C would not be the same always.Thus , they will get separated during electrophoresis . So, how can we have a common mark denoting the nucleotide number for such polynucleotides as they would be apart from each other . Can we have only sizewise separation of molecules in electrophoresis? If yes , then ,I think , such polynucleotides may have same mark as the size-difference between them seems to be lesser.

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Postby Niqqie » Fri May 20, 2005 6:38 pm

The average molecular weight of the four nucleotides is 330 daltons, you can use this average.

The individual molecular weights are:
A 313.15
C 289.13
G 329.19
T 304.19
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Postby 2810712 » Mon May 30, 2005 6:00 am

Thanks for those individual molecular wt.s ,
I calculated the diference it is much lesser , but one has to take care that not to increase the voltage to a value that would amplify this diff. , but normally this diff can be neglected.

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