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DNA analysis

Genetics as it applies to evolution, molecular biology, and medical aspects.

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DNA analysis

Postby Trishntonia » Mon Nov 27, 2006 7:17 pm

What part of blood is used for DNA analysis and why?


Thanks a bunch!!

Angel :)
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Postby rob3 » Mon Nov 27, 2006 7:25 pm

The white blood cells, red blood cells contain no DNA so cannot be used for sequencing; white blood cells do and can.
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Postby Vibrio » Mon Dec 18, 2006 4:45 am

I thought all cells contained DNA. Otherwise how could they replicate? Or do white blood cells turn to red when they age?
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Postby sachin » Mon Dec 18, 2006 5:34 pm

Vibrio wrote:I thought all cells contained DNA. Otherwise how could they replicate? Or do white blood cells turn to red when they age?


RBC do not contain DNA, and Do not replicate.
WBC are differant than those of RBC... :roll:
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Postby Oscerot » Mon Dec 18, 2006 8:32 pm

sachin_at_biog wrote:
Vibrio wrote:I thought all cells contained DNA. Otherwise how could they replicate? Or do white blood cells turn to red when they age?


RBC do not contain DNA, and Do not replicate.
WBC are differant than those of RBC... :roll:


He was told that already. He was asking how red blood cells are created if they don't contain DNA.
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Postby LilKim » Mon Dec 18, 2006 11:45 pm

Oscerot wrote:
sachin_at_biog wrote:
Vibrio wrote:I thought all cells contained DNA. Otherwise how could they replicate? Or do white blood cells turn to red when they age?


RBC do not contain DNA, and Do not replicate.
WBC are differant than those of RBC... :roll:


He was told that already. He was asking how red blood cells are created if they don't contain DNA.


..wow! easy man... be nice!
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Postby LilKim » Mon Dec 18, 2006 11:46 pm

To further clarify. MATURE human RBC's don't have a nucleus (thus, they do not contain DNA.

However, immature RBC (in the bone marrow) are nucleated.. but somehow 'lose' their nucleus upon maturation.

But, I believe bird RBC are nucleated (they contain DNA).

Hope this helps!

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Postby Vibrio » Mon Dec 18, 2006 11:55 pm

WBC are differant than those of RBC...


Yeah, I kinda knew that since like 2nd grade. so you don't have to roll your eyes at me!!

Thanks LilKim. I had forgotten that they come from bone marrow.

I did not think RBC and WBC were the same thing, I was wondering if maybe WBC turned into RBC after a period of time, but now I see they don't.
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Postby sachin » Tue Dec 19, 2006 5:36 am

May be you understand better here...

http://www.fortunecity.com/greenfield/r ... oiesis.htm
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Postby ninad » Tue Dec 19, 2006 4:05 pm

I think the chorosomes and some kinda cells are used
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Postby chenwithasterisk » Mon Jan 01, 2007 9:17 pm

well, i guess something's wrong.
i thought only mitochondria and plastids contain DNA,in eukaryotes.all the DNA is contained in nuclues?

cheers
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Postby LilKim » Tue Jan 02, 2007 3:38 am

both mitochondria and nuclei contain DNA.... mitochondrial DNA encodes most of the genes necessary for mitochondrial function (mitochondria also have ribosomes) Learn more here: http://en.wikipedia.org/wiki/Mitochondria

The cell nucleus contains DNA encoding all of the other genes necessary to make the cell a functional,living entity... additionally, the nucleus encodes a few gene necessary for mitochondrial function.
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