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The Fiber Disease

Human Anatomy, Physiology, and Medicine. Anything human!

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Postby al » Tue Dec 12, 2006 10:18 pm

i guess the thrust here, old boy, is that as you assert that you removed some "injurious posts", surely the protocol is to warn/pm the offender, to enable FREE SPEECH to thrive.
Canalong I accuse you of dirty tactics, and I put to you to defend your actions, with all eyes peeled for your sputterings.
al
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Postby canalon » Tue Dec 12, 2006 10:41 pm

Sabrina wrote:Perhaps that's why Randy isn't bothering us any more! Thanks Patrick.


No I try not to enter in your internal fights. I delete obvious insults that is all.
Patrick

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any proof. (Ashley Montague)
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Postby canalon » Tue Dec 12, 2006 10:45 pm

al wrote:i just think ur rude canalong, who are you anyway?
just come out of the bushes, like a predator...
just another morgellons day..........
i hope ur ass falls out


I am one of the moderators of this forum. I am here to make this forum work, and I am trusted by the admin to make the rules. You might not agree with what I am doing, too bad. You can always try to contact the admin or the other mods and complain, but so far the way we enforce the policy to make this forum nice and clean have always been respected by the users. If you disagree, nobody is forcing you to stay on this board.

Beside I have not deleted one single post containing what is presented here as information. I might not agree and consider that most of what is posted here is mostly irrelevant and half(at best) understood, I let you post whatever you want. The posts deleted were old post from tamtam that he is copying and pasting and replacing with "//// forward///", and a few obvious insults that have nothing to do here and do not belong to this board.

As a matter of fact I should delete most of your post because they obviously break our rules.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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Postby al » Tue Dec 12, 2006 11:02 pm

you preening sactimonious peurile peice of cow poo
the only insults are here and now
you have shown your true agenda
peace to you mate

the people heard what i said, there were no insults
Patrick, you ole brother you, I dont give a flying **** what you do
maybe we need some more illuminati-on from your esteemed self
you are a lying ludditte, a book burner, an enemy of free speech.
If your a moderator, then folks, we have more than morgellons to quake at.
I call on you to state clearly the nature of the "insults"
I proffered, I thought i was talking about Prayer, then rife machines, but you take the classic character vilification approach.
So, ban me, or back to your books, bucko, and clearly state where, before now, I've been abusive.
I care not for your sensibilities, if I offend, take it.
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Postby al » Tue Dec 12, 2006 11:08 pm

this is just a fit up

From: canalon
To: al
Posted: Tue Dec 12, 2006 10:45 pm
Subject: [ SYSTEM ] Warn
You have been warned by the admin or mods of this forum. If you get 4 warnings, you will be banned.
Insulting post

I ask any one to say when I have been abusive, apart from when i realized my posts were banned, in respect to Sabrina's question....
I reckon canolong is in it up to his neck...

ban me please


warning level:2
and rising
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Postby London » Tue Dec 12, 2006 11:09 pm

Al, I hope you don't leave but do understand if you wish to. I hope you see this on fiber optics before you go.....
________________________________

Tam Tam,

You still think that computers can't hurt us, eh? Hmmm....I do not anywhere near agree with that.

To all, take a look at this. It has the beads that MSC spoke of and yes, you betcha it is about computers.....

New Tool Speeds Up DNA Sequencing By 100 Times
DNA sequencing keeps getting faster and cheaper.
454 Life Sciences Corporation, a majority-owned subsidiary of CuraGen Corporation , today announced the publication of a new genome sequencing technique 100 times faster than previous technologies. This is the first new technology for genome sequencing to be developed and commercialized since Sanger-based DNA sequencing. 454's proprietary technology is described in the paper "Genome sequencing in microfabricated high-density picoliter reactors," in the July 31, 2005, online issue of Nature, with the print edition of the paper to follow later in the year. The technique was demonstrated by repeatedly sequencing the bacterial genome Mycoplasma genitalium in four hours, with up to and exceeding 99.99% accuracy. With a 100-fold increase in throughput over current sequencing technology, 454 Life Sciences' instrument system opens up new uses for sequencing, including personalized medicine and diagnostics, oncology research, understanding third world diseases, and providing fast responses to bioterrorism threats and diagnostics.
"It is clear that sequencing technology needs to continue to become smaller, faster and less expensive in order to fulfill the promise of personalized medicine," said Francis S. Collins, M.D., Ph.D., Director of the National Human Genome Research Institute. "We are excited that our support of sequencing technology development is yielding results and we look forward to the applications of such innovative technologies in biomedical research and, ultimately, the clinic."
In May 2004, the NHGRI awarded a grant to 454 Life Sciences to help fund the scale-up of 454 Life Sciences' technique toward the sequencing of larger genomes, starting with bacterial genomes, and to develop the Company's ultraminiaturized technology as a method to sequence routinely individual human genomes. The scalable, highly parallel system described in this article sequenced 25 million base pairs, at 99% or better accuracy, in a single four hour run. The researchers illustrated the technique by sequencing the genome of the Mycoplasma genitalium bacterium.
"Much like the personal computer opened up computing to a larger audience, this work will enable the widespread use of sequencing in a number of fields, and ultimately place machines in your doctor's office," stated Jonathan Rothberg, Ph.D., senior author and 454 Life Sciences' Founder and Chairman of the Board of Directors. "This sequencing technique, leveraging the power of microfabrication, is 100 times faster than standard sequencing methods at the start of its development cycle. We expect, as with computers, for it to get more powerful and cheaper each year, as we continue to advance and miniaturize the technology."
To repeat a frequent FuturePundit theme: Biotechnology is going to advance at the rate of computer technology because biotechnology is shifting toward the use of very small scale devices. The current cost of human DNA sequencing is in the tens of millions of dollars per person. But that high cost won't last for much longer.
The half million dollar sequencing machine uses 1.6 million tiny reaction wells in parallel.
The novel sequencing technique, designed by Jonathan M. Rothberg of 454 Life Sciences Corp. in Branford, Conn., and his colleagues, uses tiny fiber-optic reaction vessels that measure just 55 micrometers deep and 50 micrometers across--a slide containing 1.6 million wells takes up just 60 square millimeters.
The 25 million base pairs that this machine sequenced in 4 hours should be compared to the approximately 3 billion base pairs in the human genome. If the machine's ability to process 25 million base pairs in 4 hours scales up to larger genomes then the human genome would take 480 hours or 20 days on this machine. But there are additional challenges in sequencing a large genome such as breaking it down into doable pieces. Also, the genome has to be read several times to correct for errors.
This article has details on the process used by this sequencing instrument.
The 454 approach involves shearing the starting material DNA using a nebulizer. Rothberg explains: “[We] nebulize the DNA into little fragments, shake it in oil and water, so each DNA fragment goes into a separate water droplet. So instead of bacteria, we separate the DNA into drops. Then we do PCR, so every drop has 10 million copies. Then we put in a bead, drive the DNA to the bead, so instead of the cloning and robots, one person can prepare any genome.”
The DNA-covered beads are loaded into the microscopic hexagonal wells of a fiber-optic slide, which contains about 1.6 million wells. In 454’s benchtop instrument, chemicals and reagents flow over the beads in the wells. Solutions containing each nucleotide are applied in a repetitive cycle, in the order T-C-A-G. Excess reagent is washed away using a nuclease, before a fresh solution is applied. This cycle is repeated dozens of times.
The researchers see their techology following a similar pattern to the development of integrated circuits which have sped up at the rate predicted by Intel co-founder Gordon Moore with his famous Moore's Law.
“Future increases in throughput, and a concomitant reduction in cost per base, may come from the continued miniaturization of the fibre-optic reactors, allowing more sequence to be produced per unit area – a scaling characteristic similar to that which enabled the prediction of significant improvements in the integrated circuit at the start of its development cycle.”
Future iterations of their design will increase the level of parallelization while at the same time keeping costs the same per instrument or even lowering costs per instrument. So these folks have an approach that will drive down DNA sequencing costs by orders of magnitude.
Rothberg expects individual DNA sequencing for medical purposes once DNA sequencing costs fall to $20,000.
Jonathan Rothberg, board chairman of 454 Life Sciences, said the company was already able to decode DNA 400 units at a time in test machines. It was working toward sequencing a human genome for $100,000, and if costs could be further reduced to $20,000 the sequencing of individual genomes would be medically worthwhile, Dr. Rothberg said.
Another very important application of cheap DNA sequencing technology which is rarely mentioned is in social sciences. Cheap DNA sequencing will allow controlling for genetic influences on behavior in social science experiments. Most existing social science research results will be discredited by experiments that control for genetic influences. The excessive assumption of environmental influences on human behaviors and abilities will be discredited. This will lead to the disproof of key assumptions underlying beliefs of factions on both the political Left and the political Right.
http://www.futurepundit.com/archives/ca ... rates.html

and DNA sequencing speeds up............

Begun in 1990, the sequencing of the human genome was a daunting task that took some 13 years to complete. Large-scale DNA-sequencing projects are now more commonplace, so scientists are investigating new approaches in order to save time and money. A report published online by the journal Nature describes one such method that is 100 times faster than conventional ones.
The novel sequencing technique, designed by Jonathan M. Rothberg of 454 Life Sciences Corp. in Branford, Conn., and his colleagues, uses tiny fiber-optic reaction vessels that measure just 55 micrometers deep and 50 micrometers across--a slide containing 1.6 million wells takes up just 60 square millimeters. The set up allows for the amplification and sequencing of hundreds of thousands of DNA molecules simultaneously. Using light to measure the reactions, tests of the apparatus indicate that it can sequence 25 million bases in a single four-hour run with greater than 99 percent accuracy.
To compare the approach to standard procedures, one member of the research team successfully sequenced and assembled the entire genome of the parasitic bacterium Mycoplasma genitalium, which includes 580,069 base pairs, in a single run on the new machine. The results cover 96 percent of the genome with 99.96 percent accuracy. The authors conclude that the continued miniaturization of fiber optics suggests that in the future, this technique could become even faster when more sequencing wells can fit in a given area
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Postby al » Tue Dec 12, 2006 11:16 pm

thanks for your support London, Deena
See me only as a bellweather
every thing from here on in is subject to
censorship
this is group dynamics gone mad ala Lord of the Fly's
I just ask that I be remembered in the spirit of altruism, that i sought to bring.
Never mind
Where are your Jackboots, Patrick?
al
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Postby London » Tue Dec 12, 2006 11:30 pm

tell me I don't know......and this is to them....Look, I'm not the one that put this here article on the http://www......you made it, you made it go stronger and faster and burn your stupid green energy......lots of mines in ireland or there not? Look, let us go, stop this shite damn you....

Hey thank you mr rothman or whatever t.f. your name is....you suck rocks....

Fibre optic link devices for thermonuclear fusion
reactor environments


http://www.sckcen.be/people/affernandez ... leos99.pdf.

oh, why darn me, this one here blows the other away......let's all see:

http://www.454.com/downloads/enabling-t ... rticle.pdf
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Postby London » Tue Dec 12, 2006 11:42 pm

To MSC, this is for you....it's not much, but hey.....

let their arses come get me....the NSF plus a whole lot of others will be implicated.....plus, it's not so fun this fiber lined life from hell....

You A-holes.

A sensitive fibre optic pH sensor using multiple sol-gel coatings
Abstract. The fabrication and characterization of a fibre optic pH sensor based on evanescent wave absorption is presented. The unclad portion of a multi-mode optical fibre is coated with a pH sensitive dye, which is immobilized by the sol-gel route. The sensitivity of the device has been found to increase when multiple sol-gel coatings are used as the sensing region. The dynamic range and the temporal response of the sensor are investigated for two different dyes, namely bromocresol purple and bromocresol green. The performance of the device is evaluated in terms of the results obtained during actual measurements.

[PDF]
A sensitive fibre optic pH sensor using multiple sol–gel coatings

File Format: PDF/Adobe Acrobat
environments, such as deep-water analysis, chemical reactors. or wastewater monitoring. Fibre optic pH sensors have many. advantages over conventional pH ...
http://www.iop.org/EJ/article/1464-4258 ... oa1507.pdf - Similar pages

[PDF]
Fibre optic fluorometric enzyme sensors for hydrogen peroxide and ...

File Format: PDF/Adobe Acrobat
HRP in a reactor combined with fibre optic detection the feasibility of this concept ... developed enzyme reactor-fibre optic detector device [25]. ...
http://www.springerlink.com/index/U406288852636670.pdf - Similar pages
London
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Postby London » Tue Dec 12, 2006 11:49 pm

oh great- on world new tonight : WHAT IS CAUSING THE MYSTERIOUS OUTBREAKS OF E COLI?????

Hmm....COULD IT BE THE BIOFILMS THEY PUT IN THE WATER?????

DUH....... and what is causing all these mysterious staph infections????

IT IS THE PACKAGED MEAT!!!!

WORD UP!
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Postby Skytroll » Wed Dec 13, 2006 12:04 am

When we know who we are Really, we will then have reached a wonderful place.

s
Last edited by Skytroll on Sat Dec 16, 2006 8:16 am, edited 1 time in total.
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Postby canalon » Wed Dec 13, 2006 12:16 am

al wrote:this is just a fit up

From: canalon
To: al
Posted: Tue Dec 12, 2006 10:45 pm
Subject: [ SYSTEM ] Warn
You have been warned by the admin or mods of this forum. If you get 4 warnings, you will be banned.
Insulting post

I ask any one to say when I have been abusive, apart from when i realized my posts were banned, in respect to Sabrina's question....
I reckon canolong is in it up to his neck...

ban me please


warning level:2
and rising

Your warning level is still one.Detail answere in PM, but wishing someone death IS insulting.
By the way if you read the rules, you will see that we may ban you before you reach the warning level of 4...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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