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Stein's Publication

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Stein's Publication

Postby Dr.Stein » Thu Nov 23, 2006 8:54 am

The Effect of Seahorse (Hippocampus kuda Bleeker 1852) Extract On Spermatogenesis of Mice (Mus musculus L.)

Laksmindra Fitria
Laboratory of Animal Physiology, Faculty of Biology, Gadjah Mada University, Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281 Indonesia. Phone/Fax: 62-274-580839, E-mail: laksmindra.fitria@yahoo.com

ABSTRACT

For centuries, it has been believed that seahorse can be used to deal with male sexual dysfunction. However, there is still less scientific evidence to provide this myth. This research was carried out to study the effect of seahorse extract on spermatogenesis of mice. Balanced-factorial-CRD was applied for the experiment design. Sixty three-month-year-old male mice were divided into two groups. Each group was divided into six subgroups. Four subgroups were for treatment, the rest two subgroups were for controls. Seahorse was extracted using maceration method and was dissolved into distilled water to get final dose 100; 150; 200; 250 mg/kg BW. Extract was administered orally 0.5 mL/day for 34 and 67 days. Control groups were supplied with tap water or distilled water (placebos). On day 35 and 68, mice were sacrificed. Testes were taken and were processed for histological preparation in paraffin method using fixative Bouin and HE staining. Number of spermatogonia, spermatocytes, spermatids, and total spermatogenic cells were counted. Data were analyzed statistically using ANOVA and continued with LSD. Result showed that number of spermatogenic cells from all treatment groups significantly increased with increasing dose and duration of treatment (linear). In control groups, number of spermatogenic cells significantly decreased with increasing duration of treatment. Optimum treatment was obtained on dose 200 mg/kg BW, which was administered for 67 days. It was concluded that seahorse extract positively affected on increasing and maintaining spermatogenesis on mice. By consuming seahorse regularly as a daily menu could improve potential of fertility on male individual.

Keywords: seahorse, extract, mice, spermatogenesis


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Presented to International Seminar and Workshop “Marine Biodiversity and the Potential For Developing Bio-Pharmaceutical Industry in Indonesia on May 17-18 2006, Kompleks Bidakara, Jakarta.





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Postby sachin » Thu Nov 23, 2006 11:59 am

Its Nice...May be SeaHorse exttract has high sex hormone value.

But have you tested that
At what stage of maturity the seahorse was when the extract were prepared? :o

That may be point to note... :P
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Postby Dr.Stein » Fri Nov 24, 2006 4:15 am

That's the preliminary research. My current research is aimed to test the hormone level with radioimmunoassay method :)

About the seahorse, unfortunately I have no precise data of their maturity. According to the guys from the seafarming, where we culture seahorses, they always harvest them periodically, so I think they have similar age/maturity. Thank you for your input, I will try to collect better data about this ;)
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Postby Linn » Fri Nov 24, 2006 4:57 pm

People really have sea horse on the menu? :!:
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Postby Dr.Stein » Mon Nov 27, 2006 4:42 am

Yes, of course. My research is based on Chinese belief and habit of consuming seahorses for their daily menu. They believe that by eating them, even just by keeping them alive or dead (dried specimen), it could maintain their sexual performance, even their entire body health :)
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Postby Linn » Tue Nov 28, 2006 4:22 pm

Oh I thought they only dried them and then ground them up in aphrodisiac potions :oops:

It was concluded that seahorse extract positively affected on increasing and maintaining spermatogenesis on mice. By consuming seahorse regularly as a daily menu could improve potential of fertility on male individual.



So science helps confirm what the Chinese have known for centuries.

This is very interesting paper Dr Stein
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Postby Dustfinger » Fri Dec 01, 2006 11:35 am

Wow, Dr.Stein ! U have got a publication of your own ! Awesome !!!
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Postby Dr.Stein » Fri Dec 01, 2006 2:16 pm

Thank you again, Linn & Dusti :)

Actually we still have a gap between medical/scientifical drugs and traditional medicine, especially Chinese according to their phylosophy of medication.

I want to dig this Chinese phylosophy, whether it's possible to "translate" it into scientifical discussion. However I am still on the first step and need many supports here and there to realize my objective.

Thus, feel free to critize and give suggestions for methods. I would be very grateful for that! :D
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Postby nugget » Fri Dec 01, 2006 11:02 pm

This is very very interesting. I love how it is testing traditional medicine, because a lot of ways we got better when i was young was also things that we had brought down from our grandmothers and things they used to do back home.

The answer to the question may be in our faces, maybe finding some similar thread between the different "cures" for male dysfunciton, maybe looking at the different things people use as an afrodisiac may clue to a key component to helping improve spermatogenesis.

However your main hypothesis, are we looking at male sexual dysfunction in general? or particularly spermatogenesis?
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Postby Linn » Tue Dec 05, 2006 5:29 am

My grandmother had some remedies that really work and I have used them with my own family.

One thing that happened is when my granddaughter was born she had a serious eye infection that would not go away, the doctors didnt seem to be helping and I tried my grandmothers remedy which cleared it up in less than a week.
This is a subject I am very interested in :)
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