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electrophoresis

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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electrophoresis

Postby kabuto » Mon Aug 21, 2006 1:46 pm

which one is better in gel electrophoresis??
radioactive probe or non-radioactive probe
why??
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Postby mmiaosmiling » Mon Aug 21, 2006 1:59 pm

that will depend on what ur aim is and how accurate u need.
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Postby kabuto » Wed Nov 08, 2006 8:01 pm

can u explain further? :shock:
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Postby canalon » Thu Nov 09, 2006 2:57 am

You do not probe in electrophoresis... you use a probe in a southern. And then radioactivity for probe is almost religion :)
More seriously some people will defend the non-radiaoctive probes as as good as radioactive, while others will tell you that radioactive is best. In my experience both have problems and at least non-radioactive are much less trouble to handle.
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any proof. (Ashley Montague)
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Postby sachin » Thu Nov 09, 2006 4:26 am

Its best to use non radioactive probe. As intense care to handle and detect.

But non-radioactive or say "fluroscent calating" is best practiced for AGEP, PGAL etc...
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