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spleenocyte count for hybridoma production

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spleenocyte count for hybridoma production

Postby shafirm » Mon Oct 30, 2006 12:08 pm

Hi,
I prepare hybridomas, using mouse spleenocytes and myeloma cells ( one spleen to about 50,000,000 myeloma cells).
I will soon be preparing a hybridoma with rat spleenocytes, and want a protocol for counting the spleenocytes, and how many spleenocytes to how many myeloma cells?
Thanks for the help,
Milli
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Postby Dr.Stein » Tue Oct 31, 2006 6:18 am

I think you can use the regular protocol for lymphocyte counting. More cells are better to provide reservation :)
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thanks, I'll check that out

Postby shafirm » Tue Oct 31, 2006 6:48 am

thanks, I'll check that out
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