Chelex 100

[CZU]

Hi
I want to use Chelex 100 resin to extact DNA but in catalogue i found two kind of this reagent (with dry mesh size 100-200 and 200-400). Do you know practical differences between these reagents?

[sachin chandraker]

Chelex is great for DNA extraction for difficult samples often seen in forensic type work and inexpensive. This is from a Biorad leaflet..
Chelex 100 molecular biology grade resin is a highly pure, pipettable, nuclease and ligase inhibitor-free chelating resin specifically designed and certified for extraction of PCR-ready template DNA. Chelex 100 molecular biology grade resin accommodates the stringency required for a PCR-quality product by ensuring the complete removal of PCR inhibitors (contaminating metal ions that catalyze the digestion of DNA)...

Its a size thing as the resin has many applications and most people use the larger 200-400 mesh for DNA work. I am sure someone out there has got the real reasons but in practical terms go for the larger one.

sachin chandraker
source: BW Parky

[ceydakansu]

Hi,
I have ordered chelex 100 for dna isolation, but could not succeeded. It is probably my samples were gr+ bacteria. Has anyone have experience working with chelex for isolation of dna?