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bidimensional electrophoresis

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bidimensional electrophoresis

Postby MrMistery » Mon Jun 19, 2006 1:50 pm

Ok, so how the heck does bidimensional protein electrophoresis work? It's in the sillabus for IBO and i can't find anyhting decent about it. The Romanian 2006 olympic team would appreciate any help, please... :lol: :lol: :lol:
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Postby LilKim » Mon Jun 19, 2006 6:26 pm

i'm not sure... but, are you refering to 2-D gel electrophoresis?
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Postby MrMistery » Mon Jun 19, 2006 7:57 pm

YES, YES, YES! Tell me if you know anything. Save my soul!!!
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Postby LilKim » Mon Jun 19, 2006 8:19 pm

Ok.. this topic really confused me. But, i'll consult with my "cell bio" notebook when I go home... and I'll see if I can try to explain (and give you a reference)
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Postby oppox » Mon Jun 19, 2006 10:57 pm

google on it I found alot on the subject. I typed in: electrophoresis SDS 2d :)
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Postby canalon » Tue Jun 20, 2006 2:14 am

The Biorad site is full of tips and protocol. If you keep in mind they are just a supplier among others it can be interesting.
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Postby LilKim » Tue Jun 20, 2006 2:32 am

So I consulted my notes and the 2D electrophoresis we discussed in class had to do with electrophoresis of Replicating DNA (at an origin of replication/replication fork).

During the first electrophoresis, the DNA fragments (Forks) were run through a gel that simply separated the DNA by size.

During the second electrophoresis the 'electrodes' move the sample in another direction (perpendicular to the first electrophoresis). Thee second electrophoresis seeparates thee DNA forks based on their shape (ie. Big forks Vs. littlee forks)

I also checked wikipedia (partially read the article).. http://en.wikipedia.org/wiki/Two-dimens ... rophoresis

Also, i went back to my notes to find you a reference. But GOSH.. thee paper that I read (that was based on 2D elec. ) was waaaay too complicated. (so i'll spare you the painful death)

anyways, hope this helps (please let mee know if you need further clarification!)

- kim
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Postby MrMistery » Tue Jun 20, 2006 5:41 am

Thanx very very much. If i get the gold medal at IBO(which i won't anyway :lol: ) i'll be sure to thank the forum :)
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