[Seeking advise] About locating a sequence from vector

[bbs_r]

Hi there.

Since the result from my sequencing reaction is unsatisfactory, I would like to locate the target sequence via comparing my sequence with an official vector sequence. So that I might get an idea about where the target/inserted sequence could roughly be.

Does it sound like a good idea? or do you have any better ideas if you were me?

Thank you very much!

PS. Since the experimental period of my project is passed, I can't do any more experiments

[canalon]

The complete sequence of vectors is usually available through the manufacturer website. If you used PCR II from invitrogen (as another post suggests) it is definitely on thei website.

[dharma]

i think u can use any vector databases for the specific organisms lide epd , mdb, ddb.