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[Seeking advise] About locating a sequence from vector

Everything on bioinformatics, the science of information technology as applied to biological research.

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[Seeking advise] About locating a sequence from vector

Postby bbs_r » Thu Apr 06, 2006 12:07 pm

Hi there.

Since the result from my sequencing reaction is unsatisfactory, I would like to locate the target sequence via comparing my sequence with an official vector sequence. So that I might get an idea about where the target/inserted sequence could roughly be.

Does it sound like a good idea? or do you have any better ideas if you were me?

Thank you very much!

PS. Since the experimental period of my project is passed, I can't do any more experiments
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Postby canalon » Thu Apr 06, 2006 6:37 pm

The complete sequence of vectors is usually available through the manufacturer website. If you used PCR II from invitrogen (as another post suggests) it is definitely on thei website.
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Postby dharma » Mon May 22, 2006 11:03 am

i think u can use any vector databases for the specific organisms lide epd , mdb, ddb.
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