Cox I gene

by **HELISA** » Sat Apr 01, 2006 12:37 am

Hi, recently I was searching some information about cox I sequence which encodes a cytochrome c oxidase subunit I of respiratory Complex IV. I have found the information that the sequence of this gene is highly conserved among different plant species. Why is that?

by **2810712** » Sat Apr 01, 2006 2:46 pm

i think its the 'moving' sub-unit...
more no. of adaptations required more of the mutations produced would not suvive... most of DNA requires to be com\nserved or the possible no. of non-affecting [or that of variations] changes becomes lesser...
So 'C' is just so...so...not changing...but amaizingly adjusting!

hrushikesh

by **HELISA** » Sat Apr 01, 2006 11:54 pm

Hmm, I don't get it...maybe somebody else will help me?

by **LilKim** » Sun Apr 02, 2006 1:38 am

I am not sure... but If I remember my cell bio. class (from several years ago) Cytochrome C is a protien that's in the inner membrane of mitochondria .. and I beleive that it's necessary for "making" energy and life ...

Because during apoptosis (programmed cell death) this cytochrome is "expelled" from the mitochondria... and this is basically the point of no return and the cell will die.....

So I guess.. it's necessary for mitochondrial function.. which is necessary for cellular survival... (plant and animal)

hope this helps(?)

by **2810712** » Sun Apr 02, 2006 6:43 am

much of cytochrome c oxidaaase sequence is conserved-->no changes are allowed in much of its sequence--> that much of it is adapted to some thing which is conserved that's why it needs to be conserved...

for the saake of explaining, consider a 10AA protein...
for the survival of the species, it is required to have 3 basic AAs on positions 1, 4 and 8... other sequence may be anything [ just for the sake of ease]...so 3 adaptations are there... and they are distributed among 3 sites in protein... Those 3 sites will be conserved more than others...
but if there r 3 adaptations but all distributed among just one AA [ eg. presence of CH3 group, ring and -N= group or imine group etc,. then even if the no. of adaptations is same, 3 , only that specific siteto which all these r asigned will be conserved... so the conserved nature depends upon the distribution of adaptations along with the no. of adaptation...

And the adaptations may be arising in the case of CytoCox as it has to have many conserved [ essential for survival] qualities and those too distributed all along its sequence...

Hope this has helped a little more...

hrushikesh

by **HELISA** » Mon Apr 03, 2006 12:57 pm

Thank you very much. Now I get it ! :-D

by **2810712** » Mon Apr 03, 2006 2:30 pm

welcome

this was a general explanation... but what are those adaptations in case of cytochrome cox i don't know...
please tell it if you know...i'm curius...

hrushikesh

by **HELISA** » Mon Apr 10, 2006 8:15 pm

no clue...
but I have been comparing the sequence of coxI gene from Coix lacrymajobi with the same sequence from Maize and I found some slight changes in Coix lacrymajobi sequence. Actually there are only 3 transition mutations, which doesn't affect the amino acid sequence...so it looks like there are some changes in coxI sequence but still they are not adaptive...
All the best,

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