SSR or Microsatellite Markers

[Master DRe]

Hi everyone,
I'm presently developing SSR markers on conifer species and I'm not able to remove the stutter bands. I have tried to increase the temp, decrease the primer concentration, modified the [MgCl2] etc...
Do you have an advice for me??
Thanks

[HELISA]

Change the polymerase. Sometimes, enzymes have some kind of contaminations of bacterial DNA which can result in additional bands on your gel. All the best.