Rho

[Christina]

Does anyone know what the difference between rho+ , rho-, and rhoº?

[kiekyon]

do you mean rh+ and rh-

[sdekivit]

you mean rho as the termination factor in transcription ?

[Ajay]

Do u mean the initiation transcription factor which binds with the Rna polymerase?

[sdekivit]

Do u mean the initiation transcription factor which binds with the Rna polymerase?

hmmm i only know the rho protein that helps terminate the transcription of some genes, not the initiation of transcription

[Ajay]

Yeah Rho actually helps in termination of transcription, but it gets attached during the initiation stage of transcription to the Rna polymerase enzyme and gets released during termination. Ok there are two types of terminators.

1) Rho dependent
2) Rho independent



1) Rho dependent:

Termination with presence of Rho-factor. Rho is a protein with six subunits. It only binds to a specific sequence.

2) Rho independent:

They are called intrinsic terminators. They consist of two sequences, a short inverted repeats having 20 nucleotides, followed by a stretch of 8 AT base pairs.

AJAY

[sdekivit]

the last pathway involves a hairpin loop in the DNA

[Christina]

I was actually looking at the rho (not the protein) and how it was associated with mitochondrial DNA. I think that it is in referance to wild type and mutant but I am not exactly sure.