Yeast fermentative characteristics

[Nithin]

Greetings,
I am Nithin doing final year Biotechnology and Genetic engineering. I am currently diong a project on fermentation. Can anyone please tell me how to improve yeast fermentative characteristics genetically?? Yeast acts on Mollases to produce ethanol and Carbod-di-oxide. I want to increase the production of ethanol.

[fushxmy]

Delete FPS1,GPD1,GPD1 .overexpress GLT1 or GLN1.

[Nithin]

Any idea how to do it!!!
I was also suggested to over express SUC2 and YPLO88W.
Anymore great ideas.

[12345]

How to identify the various genes responsible for various activities. And after identifying them how to cause an overexpression of a particular gene. Please??

[canalon]

For the first step, microarrays, when available, are a good way to go. Otherwise a lot tedious analysis of expression level using things like RT-PCR and substractive hybridization for example.

Second step, clone it in a vector with a controled promoter is probably the simple answer. But if you want to do it in physiological conditions, I have no idea.