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Rho

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Rho

Postby Christina » Sat Feb 18, 2006 8:35 pm

Does anyone know what the difference between rho+ , rho-, and rhoº?
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Postby kiekyon » Sat Feb 25, 2006 8:55 am

do you mean rh+ and rh-
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Postby sdekivit » Sat Feb 25, 2006 11:19 am

you mean rho as the termination factor in transcription ?
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Re:Rho

Postby Ajay » Sat Feb 25, 2006 5:40 pm

Do u mean the initiation transcription factor which binds with the Rna polymerase?
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Re:Rho

Postby sdekivit » Sat Feb 25, 2006 5:44 pm

Ajay wrote:Do u mean the initiation transcription factor which binds with the Rna polymerase?


hmmm i only know the rho protein that helps terminate the transcription of some genes, not the initiation of transcription :roll: :?:
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Re: Rho

Postby Ajay » Sun Feb 26, 2006 5:51 pm

Yeah Rho actually helps in termination of transcription, but it gets attached during the initiation stage of transcription to the Rna polymerase enzyme and gets released during termination. Ok there are two types of terminators.

1) Rho dependent
2) Rho independent



1) Rho dependent:

Termination with presence of Rho-factor. Rho is a protein with six subunits. It only binds to a specific sequence.

2) Rho independent:

They are called intrinsic terminators. They consist of two sequences, a short inverted repeats having 20 nucleotides, followed by a stretch of 8 AT base pairs.

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Postby sdekivit » Sun Feb 26, 2006 9:42 pm

the last pathway involves a hairpin loop in the DNA :)
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Postby Christina » Wed Mar 08, 2006 7:35 pm

I was actually looking at the rho (not the protein) and how it was associated with mitochondrial DNA. I think that it is in referance to wild type and mutant but I am not exactly sure.
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